Antiviral & Antimicrobial Countermeasures Discovery and Development Core

项目摘要 建议的抗病毒和抗微生物对策发现和开发核心(在此 A2CD2C)将促进以发现和/或开发为目标的生物医学研究 预防或治疗人类生物安全3级(BSL-3)病原体或 要求选择药剂控制的BSL-2病原体。A2CD2C将使用最先进的技术支持这项研究 小分子设备(1)筛选、(2)成像、(3)液相色谱-质谱仪 (LC MS/MS)服务和(4)组织学。核心将被安置在UTHSC区域生物遏制中心内 实验室(RBL)在BSL-3空间。A2CD2C的重要之处在于它提供了高效、集成的 全面的平台，将促进从最初的发现阶段到临床前的研究 在BSL-3安全壳内的发展。A2CD2C在RBL网络中是独一无二的，其重点是提供 对BSL-3感染和处理的动物组织进行LC-MS研究，以提供急需的信息 治疗药物在感染部位的暴露，从而提供重要的药效学信息 临床前评估所需的。 A2CD2C的主要使命是维护、改进和扩展我们在药物方面的RBL支持服务 发现和发展，重点是药物药效学方面的如何和多少药物 暴露会影响病原体的存活。我们在此提出了三个目标：目标1.保持 药物发现和开发中的A2CD2C支持服务；目标2.改善A2CD2C支持服务 在药物发现和开发方面；以及目标3.扩展药物成像方面的A2CD2C支持服务 发现和发展。

PROJECT ABSTRACT The proposed Antiviral & Antimicrobial Countermeasures Discovery & Development Core (herein abbreviated as A2CD2C) will facilitate biomedical research targeted toward the discovery and/or development of countermeasures for prophylactic or therapeutic treatment of human biosafety level 3 (BSL-3) pathogens or BSL-2 pathogens that mandate select agent control. The A2CD2C will enable this research using state-of-the-art equipment for small molecule (1) screening, (2) imaging, (3) liquid chromatography-mass spectrometry (LCMS/MS) services and (4) histology. The core will be housed within the UTHSC Regional Biocontainment Laboratory (RBL) in BSL-3 space. The A2CD2C is significant in that it provides an efficient, integrated, comprehensive platform that will facilitate studies from the initial discovery phase through pre-clinical development within BSL-3 containment. The A2CD2C is unique within the RBL network in its focus on providing LC-MS studies for infected, treated animal tissues in BSL-3 to provide much needed information of the site of exposure of the therapeutic at the site of infection and thereby providing important pharmacodynamic information required for preclinical assessment. The major mission of the A2CD2C is to maintain, improve and expand our RBL support services in drug discovery and development with a focus on drug pharmacodynamics in terms of how and how much drug exposure impacts pathogen survival. We propose three objectives to achieve herein: Objective 1. To maintain A2CD2C support services in drug discovery and development; Objective 2. To improve A2CD2C support services in drug discovery and development; and Objective 3. To expand A2CD2C support services in imaging in drug discovery and development.