UTHSC RBL Administrative Supplement For Antiviral Discovery & Development

UTHSC RBL 抗病毒药物发现行政补充

• 批准号: 10636200
• 负责人: Colleen B Jonsson
• 金额: $ 202.19万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10636200
• 关键词: Administrative Supplement; Air; Air Movements; Animals; Area; Automation; Basic Science; Biological Assay; Bioluminescence; Biomedical Research; COVID-19; Cells; Chills; Clinical Research; Complement; Coupled; Data; Development; Disease; Equipment; Failure; Flow Cytometry; Fluorescence; Fostering; Functional disorder; Funding Opportunities; Future; Glycols; Goals; Hamsters; Health Sciences; Heating; Image; Immune response; Immunologics; Infection; Infectious Agent; Infrastructure; Laboratories; Lighting; Logic; Longevity; Maintenance; Manufacturer Name; Methods; Mission; Modernization; Monitor; Mus; Optics; Plants; Plaque Assay; Reaction Time; Recovery; Research; Research Personnel; Services; Structure; System; Tennessee; Therapeutic; Time; Translational Research; Tube; Universities; Update; Viral; Water; X-Ray Computed Tomography; animal imaging; biodefense; confocal imaging; design; emerging pathogen; exhaust; good laboratory practice; heat exchanger; high efficiency particulate air filter; imaging capabilities; imaging modality; imaging system; infectious disease treatment; operation; pathogen; pre-clinical; pressure; research and development; software systems; therapeutic development; trend; vaccine development; whole animal imaging

Project Summary The University of Tennessee Health Sciences Center (UTHSC) proposes to upgrade facilities and broaden research services in the existing UTHSC Regional Biocontainment Laboratory (RBL) located in Memphis, TN to support internal and external investigators in basic and translational research with emerging pathogens and select agents utilizing small animal. In Aim 1, upgrading the hardware and building automation system software that has been in place since 2009 is our top priority with this funding opportunity and will allow the UTHSC RBL to operate continuously well into the future. In Aim 2, we request support to expand existing infrastructure in order to use our current facility to its design capacity with additional caging and racks. Importantly, a major component of our plan is to upgrade our vivarium to house the Provantis Instem to provide support for preclinical services. In Aim 3 and 4, we request equipment for whole animal imaging and immunological analyses to strengthen our ability to meet the research service demands in terms of endpoints available to users; both internally and externally. In summary, this proposal seeks to: (1) upgrade and replace outdated or obsolete components of the building management system; (2) expand infrastructure for small animals other than mice, (3) upgrade current animal imaging capabilities and dedicate one animal biosafety level 3 (ABSL3) laboratory for the IVIS/CT imaging system, (4) enable preclinical services with a dedicate ABSL-3 area for Good Laboratory Practice (GLP) monitoring; and (5) equipment to enable immunological analyses currently not available (elispot) and replace an obsolete flow cytometry equipment with a spectral flow cytometer.

项目摘要 田纳西大学健康科学中心（UTHSC）建议升级设施， 位于田纳西州孟菲斯的现有UTHSC区域生物防护实验室（RBL）的研究服务， 支持内部和外部研究人员对新兴病原体进行基础和转化研究， 选择利用小动物的制剂。在目标1中，升级硬件和楼宇自动化系统软件 自2009年以来一直存在的是我们的首要任务与此资金的机会， RBL将在未来持续运营。在目标2中，我们请求支持扩展现有基础设施 为了使用我们现有的设施，其设计能力与额外的笼子和机架。重要的是，一个主要 我们计划的一部分是升级我们的动物园，以容纳Provantis Instem，为临床前研究提供支持。 服务在目标3和4中，我们要求提供用于整体动物成像和免疫分析的设备， 加强我们的能力，以满足研究服务的需求方面的端点提供给用户; 内部和外部。概括而言，本建议旨在：（1）更新和更换过时或过时的 建筑物管理系统的组成部分;（2）扩大除老鼠以外的小动物的基础设施， (3)提升现有的动物成像能力，并专门设立一个动物生物安全3级（ABSL 3）实验室 对于IVIS/CT成像系统，（4）通过专门的ABSL-3区域实现临床前服务，用于良好实验室 实践（GLP）监测;和（5）目前无法进行免疫学分析的设备（elispot） 用光谱流式细胞仪取代陈旧的流式细胞仪。