Molecular Basis and Regulatory Mechanisms of Exosome Secretion
外泌体分泌的分子基础和调控机制
基本信息
- 批准号:10792769
- 负责人:
- 金额:$ 17.91万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-05-01 至 2026-04-30
- 项目状态:未结题
- 来源:
- 关键词:Administrative SupplementAffectBiogenesisCancer BiologyCell secretionCellsCellular biologyDevicesDiameterEquipmentFluorescenceFunctional disorderFundingGoalsGrantImmunosuppressionLabelLasersMalignant NeoplasmsMarketingMeasuresMessenger RNAMolecularNerve DegenerationOncogenicParentsPlayProcessRegulationResearchRoleSignal TransductionSignaling ProteinSystemcancer cellexosomeexperimental studyextracellular vesiclesmeetingsnanoparticleparticletooltrafficking
项目摘要
ABSTRACT
Exosomes are small extracellular vesicles secreted by cells. Exosomes carry bioactive molecules such as
signaling proteins and mRNAs, and potently affect the pathophysiology of their recipient cells. The project funded
by my R35 grant “Molecular Basis and Regulatory Mechanisms of Exosome Secretion” (R35 GM141832) aims
to understanding the molecular mechanisms that control the biogenesis and secretion of exosomes, and the
regulation of exosome biogenesis by oncogenic signaling. Due to the small size (30-120 nm in diameter) of
exosomes, a nanoparticle tracking analysis (NTA) equipment is essential for the proposed study. The NTA
equipment carries out functions ranging from quantification of exosome number, measuring exosome diameters,
to fluorescence-based cargo labeling and exosome sub-population identification. Without these basic exosome
characterizations, most of our proposed experiments cannot be carried out.In this proposal, I apply for
Administrative Supplements for Equipment fund (NOT-GM-22-017) for the purchase of a ZetaView® x30 dual
laser multiple parameter NTA device to replace our broken equipment, NanoSight N300. We plan to purchase a
ZetaView®x30 from Particle Metrix, Inc. rather than the NanoSight NS300 from Malvin, Inc. because
ZetaView®x30 is a leading NTA equipment in the market with features meeting our research needs, whereas
the old NanoSight NS300 system has a number of deficiencies revealed over these years. In fact, ZetaView
system is the only NTA device that allow us to characterize exosomes with precision, and is indispensable for
the accomplishment of the goals proposed in the parent R35 grant.
摘要
外泌体是由细胞分泌的小的细胞外囊泡。外泌体携带生物活性分子,如
信号蛋白和mRNA,并有力地影响其受体细胞的病理生理学。资助的项目
通过我的R35资助“外泌体分泌的分子基础和调控机制”(R35 GM 141832),
了解控制外泌体的生物发生和分泌的分子机制,
通过致癌信号调节外泌体生物发生。由于小尺寸(直径30-120 nm),
外泌体,纳米粒子跟踪分析(NTA)设备是必不可少的拟议研究。新技术评定
设备执行的功能范围从外泌体数量的定量,测量外泌体直径,
涉及基于荧光的货物标记和外来体亚群鉴定。如果没有这些基本的外泌体
由于这些特性,我们提出的大多数实验都无法进行。在这个建议中,我申请
用于购买ZetaView® x30 dual的设备基金(NOT-GM-22-017)的行政补充
激光多参数NTA设备,以取代我们破碎的设备,NanoSight N300。我们计划购买一个
ZetaView®x30,来自Particle Meetings,Inc.而不是来自Malvin公司的NanoSight NS 300。因为
ZetaView®x30是市场上领先的NTA设备,其功能满足我们的研究需求,而
旧的NanoSight NS 300系统在这些年中暴露出许多缺陷。事实上,
该系统是唯一一种允许我们精确表征外泌体的NTA设备,并且是不可或缺的,
实现R35补助金中提出的目标。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting.
通过异位靶向确定囊泡束缚中外囊的功能。
- DOI:10.1007/978-1-0716-2209-4_6
- 发表时间:2022
- 期刊:
- 影响因子:0
- 作者:Mei,Kunrong;Liu,Di-Ao;Guo,Wei
- 通讯作者:Guo,Wei
Double Digital Assay for Single Extracellular Vesicle and Single Molecule Detection.
- DOI:10.1002/advs.202303619
- 发表时间:2023-11
- 期刊:
- 影响因子:15.1
- 作者:Reynolds, David E.;Pan, Menghan;Yang, Jingbo;Galanis, George;Roh, Yoon Ho;Morales, Renee-Tyler T.;Kumar, Shailesh Senthil;Heo, Su-Jin;Xu, Xiaowei;Guo, Wei;Ko, Jina
- 通讯作者:Ko, Jina
Modeling the Cryo-EM Structure of the Exocyst Complex.
模拟外囊复合体的冷冻电镜结构。
- DOI:10.1007/978-1-0716-2639-9_16
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Mei,Kunrong;Guo,Wei
- 通讯作者:Guo,Wei
Immunosuppressive Extracellular Vesicles in CLL.
CLL 中的免疫抑制细胞外囊泡。
- DOI:10.1158/2643-3230.bcd-22-0161
- 发表时间:2023
- 期刊:
- 影响因子:11.2
- 作者:Zhong,Wenqun;Guo,Wei
- 通讯作者:Guo,Wei
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
WEI GUO其他文献
WEI GUO的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('WEI GUO', 18)}}的其他基金
A physical sciences approach to investigate the role of exosomes in metastatic progression
研究外泌体在转移进展中的作用的物理科学方法
- 批准号:
10533613 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
Targeting exosomal PDL1 to improve immunotherapy
靶向外泌体 PDL1 改善免疫治疗
- 批准号:
10268744 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
A physical sciences approach to investigate the role of exosomes in metastatic progression
研究外泌体在转移进展中的作用的物理科学方法
- 批准号:
10689255 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
A physical sciences approach to investigate the role of exosomes in metastatic progression
研究外泌体在转移进展中的作用的物理科学方法
- 批准号:
10273891 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
Molecular Basis and Regulatory Mechanisms of Exosome Secretion
外泌体分泌的分子基础和调控机制
- 批准号:
10397628 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
A physical sciences approach to investigate the role of exosomes in metastatic progression
研究外泌体在转移进展中的作用的物理科学方法
- 批准号:
10737763 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
A physical sciences approach to investigate the role of exosomes in metastatic progression
研究外泌体在转移进展中的作用的物理科学方法
- 批准号:
10737764 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
A physical sciences approach to investigate the role of exosomes in metastatic progression
研究外泌体在转移进展中的作用的物理科学方法
- 批准号:
10533581 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
Molecular Basis and Regulatory Mechanisms of Exosome Secretion
外泌体分泌的分子基础和调控机制
- 批准号:
10205398 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
Molecular Basis and Regulatory Mechanisms of Exosome Secretion
外泌体分泌的分子基础和调控机制
- 批准号:
10614961 - 财政年份:2021
- 资助金额:
$ 17.91万 - 项目类别:
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 17.91万 - 项目类别:
Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 17.91万 - 项目类别:
Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
- 批准号:
23K00129 - 财政年份:2023
- 资助金额:
$ 17.91万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
- 批准号:
2883985 - 财政年份:2023
- 资助金额:
$ 17.91万 - 项目类别:
Studentship