Request for administrative supplement to purchase a confocal microscope for project GM139971

申请行政补充，为项目 GM139971 购买共焦显微镜

• 批准号: 10792740
• 负责人: TIMOTHY L MEGRAW
• 金额: $ 20万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10792740
• 关键词: Adipocytes; Administrative Supplement; Animals; Architecture; Basement membrane; Binding; Biochemistry; Biological Assay; Cell physiology; Cells; Cellular biology; Centrosome; Collagen; Complex; Confocal Microscopy; Cytoskeleton; Data; Development; Disease; Drosophila genus; Dynein ATPase; Endosomes; Equipment; Fat Body; Funding; Future; Generations; Goals; Grant; Homeostasis; Interphase Cell; Knowledge; Location; Maps; Measures; Membrane Proteins; Microscope; Microtubule-Organizing Center; Microtubules; Modeling; Molecular; Molecular Structure; Motor; Nature; Nuclear; Organ; Organism; Orthologous Gene; Physiology; Polymerase; Positioning Attribute; Printing; Process; Protein Isoforms; Publications; Publishing; Radial; Regulation; Research; Role; Structure; Surface; Testing; Tubulin; Vesicle; Work; aged; cell type; experimental study; frontier; human disease; infancy; instrument; member; ninein; novel; programs; recruit; synergism; trafficking

Our current project (R01 GM139971) is focused on elucidating the mechanisms by which non-centrosomal microtubule-organizing centers (ncMTOCs) function. This is important because many, if not most, non-dividing cells in the organism rely on ncMTOCs rather than the centrosome, the better understood animal MTOC. Considered a new frontier in cytoskeleton research, ncMTOCs are diverse in their structures, subcellular locations, and mechanisms of microtubule assembly. My lab has discovered three distinct ncMTOCs in the past few years, and in this project we focus on discerning the mechanisms by which a perinuclear MTOC in Drosophila adipocytes is assembled and by which it controls microtubule assembly. To achieve the aims of this grant and future projects, confocal microscopy is needed to execute and evaluate experiments and is the most critical core piece of equipment for my research program. The general and ongoing need for intensive confocal microscopy use is reflected in all of our publications in the past 13 years, where we have had ready access to a Nikon A1 confocal microscope housed adjacent to our lab. This instrument is used daily by members of my lab and is now aged, slow and in disrepair. A supplement to fund a new confocal microscope is necessary to assure progress with this project and allow us to retire the current instrument.

我们目前的项目(R01GM139971)致力于阐明这种机制 非中心体微管组织中心(NcMTOCs)通过其发挥作用。 这一点很重要，因为在有机体中，即使不是大多数，也有许多不分裂的细胞 依赖于ncMTOCs，而不是中心体，后者是更好地理解动物 MTOC。NcMTOCs被认为是细胞骨架研究的新前沿 它们的结构、亚细胞位置和微管机制各不相同 集合。在过去的几年里，我的实验室发现了三种不同的ncmtoc， 在这个项目中，我们专注于辨别一种 果蝇脂肪细胞中的核周MTOC被组装，并通过它 控制微管装配。为了实现这笔赠款和未来的目标 项目中，需要共聚焦显微镜来执行和评估实验 是我的研究项目中最关键的核心设备。这个 对密集共焦显微镜使用的普遍和持续的需求反映在 在过去的13年里，我们可以随时访问我们的出版物 尼康A1共聚焦显微镜就在我们实验室的隔壁。这件乐器是 由我的实验室成员每天使用，现在已经老化、缓慢和年久失修。一个 为了确保进展，有必要为新的共焦显微镜提供资金补充 这个项目，并允许我们淘汰目前的仪器。

项目成果

Coordination of Zika Virus Infection and Viroplasm Organization by Microtubules and Microtubule-Organizing Centers.

• DOI: 10.3390/cells10123335
• 发表时间: 2021-11-27
• 期刊: Cells
• 影响因子: 6
• 作者: Buchwalter RA;Ogden SC;York SB;Sun L;Zheng C;Hammack C;Cheng Y;Chen JV;Cone AS;Meckes DG Jr;Tang H;Megraw TL
• 通讯作者: Megraw TL