A Shimadzu micro-scale HPLC system for analysis of nanobody repertoires

项目摘要/摘要 骆驼VHH抗体或纳米抗体是一类引人注目的生物医学应用的新抗体。 纳米体具有尺寸小、折叠牢固、稳定性高等特点。尽管它们体积很小，而且 单体结合，它们保持了对抗原结合的高亲和力和选择性。此外，纳米体还可以 很容易从微生物中以低成本生产。它们是生物工程的理想模块蛋白，并且具有 在促进疾病诊断和治疗方面显示出很大的潜力。 在这里，我们建议开发集成技术来促进纳米体的发现和表征。在……里面 同时，我们将应用我们的方法和工具来实现生物医学发现和治疗。在……里面 本补充申请，我们要求资金获得灵敏的高效液相装置(岛津高效液相Nexera 40) 支持我们提出的与纳米实体鉴定以及生物物理、结构和功能相关的科学 刻画。

PROJECT SUMMARY/ABSTRACT Camelid VHH antibodies or Nanobodies are a compelling new class of antibodies for biomedical applications. Nanobodies are characterized by small size, robust fold, and high stability. Despite their small size and monomeric binding, they retain high affinity and selectivity for antigen binding. In addition, nanobodies can be readily produced with low cost from microbes. They are ideal modular proteins for bioengineering and have shown promising potential in facilitating disease diagnosis and therapy. Here we propose to develop integrative technologies to facilitate nanobody discovery and characterization. In parallel, we will apply our methods and tool nanobodies to enable biomedical discoveries and therapeutics. In this supplement application, we request funds to obtain a sensitive HPLC device (Shimazu HPLC Nexera 40) to support our proposed science related to nanobody identification and biophysical, structural and functional characterizations.