权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Intracellular cargo transport in Toxoplasma gondii

弓形虫的细胞内货物运输

基本信息

批准号：
10795244
负责人：
Aoife Heaslip
金额：
$ 18.47万
依托单位：
UNIVERSITY OF CONNECTICUT STORRS
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2025-06-30
项目状态：
未结题

项目摘要

PROJECT SUMMARY Intracellular cargo transport is a vital and ubiquitous cellular process that occurs in all eukaryotic cells. The research focus of the parent grant is to understand the cargo transport mechanism in the protozoan parasite Toxoplasma gondii. To answer these questions my research group uses a combination of cell biology and biophysical techniques and a huge proportion of the data generated in my laboratory is microscopy based. To date we have used a DeltaVision Elite microscope. However, production of this microscope was discontinued in 2019. Many replacement parts are no longer available. Repair and service of the DeltaVision has become increasingly difficult in the last 12 months. We have experienced significant delays in finding replacement parts, which has led to decreased productivity for members of my research team. In the event that a defective microscope part cannot be replaced, all progress on our research projects would stall. With this equipment supplement, we propose to replace the DeltaVision with a Ti2 system from Nikon that has improved functionality. Briefly, the Nikon system has a customizable stage that will be adapted to include a microfluidics system, facilitating the implementation of new in vitro assays and analysis pipelines. In addition, Ti2 can accommodate both confocal and high-resolution applications at either the time of installation or as future upgrades. The Ti2 has an industry leading field of view and increased acquisition speeds. The 4th generation perfect focus system and the ThermoBoxTM will enable long-term multiday imaging experiments, which are currently not feasible. Our work critically depends on a reliable and serviceable microscope. Purchase of the new Ti2 Nikon system will allow us to continue the progress on understanding the cargo transport mechanisms in T. gondii and expand our repertoire of experimental approaches.

项目摘要细胞内货物运输是一个重要的和普遍存在的细胞过程，发生在所有真核细胞。的本基金的研究重点是了解原生动物寄生虫的货物运输机制。弓形虫为了回答这些问题，我的研究小组结合了细胞生物学和生物物理技术和在我的实验室产生的数据的很大一部分是基于显微镜。到我们使用DeltaVision Elite显微镜。然而，这种显微镜的生产被停止了。 2019年许多替换零件不再可用。DeltaVision的维修和服务已经成为在过去的12个月里越来越困难。我们在寻找替代品方面经历了重大延误零件，这导致了我的研究团队成员的生产力下降。如果缺陷如果显微镜的零件无法更换，我们的研究计划便会停滞不前。对这个设备作为补充，我们建议用尼康的Ti2系统取代DeltaVision，功能.简单地说，尼康系统具有可定制的载物台，其将适于包括微流体系统，促进新的体外测定和分析管道的实施。此外，Ti2可以无论是在安装时还是将来，升级. Ti2具有行业领先的视野和更快的采集速度。第4代完美的对焦系统和ThermoBoxTM将实现长期多日成像实验，目前不可行。我们的工作关键取决于可靠和耐用的显微镜。购买新的Ti2尼康系统将使我们能够继续了解货物运输机制的进展于T.并扩大我们的实验方法。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Toxoplasma gondii actin filaments are tuned for rapid disassembly and turnover.

弓形虫肌动蛋白丝经过调整，可快速拆卸和周转。

DOI：
10.1101/2023.08.29.555340
发表时间：
2023
期刊：
bioRxiv : the preprint server for biology
影响因子：
0
作者：
Hvorecny,KelliL;Sladewski,ThomasE;DeLaCruz,EnriqueM;Kollman,JustinM;Heaslip,AoifeT
通讯作者：
Heaslip,AoifeT

Dense granule biogenesis, secretion, and function in Toxoplasma gondii.