Investigating the effect of alcohol exposure on human cortical development using a 3D in vitro model

项目摘要 本项目是根据PA-20-272（现有NIH赠款的行政补充， 合作协议（家长管理支持临床试验可选）。PI处于无成本延长期 她的F32奖学金，部分由前几年结转的资金支持。PI成功 在确定的促进分化的培养基中产生人iPSC衍生的皮质球状体， 研究了酒精对早期大脑发育的影响。我们已经评估了乙醇对 通过监测细胞死亡、形态学变化、细胞增殖和细胞周期， 祖细胞群，强调在细胞水平上球状体中的外部放射状胶质样细胞。 该拟议的管理补充将允许PI完成获得的RNA测序分析 为将来的手稿做准备。这一行政补充还将提供必要的 资源允许有序地完成父项目。

Project summary This project is prepared in response to PA-20-272 (Administrative Supplements to Existing NIH Grants and Cooperative Agreements (Parent Admin Supp Clinical Trial Optional). The PI is in no cost extension period of her F32 fellowship, partially supported by funds carried forward from previous years. The PI successfully generated human iPSC-derived cortical spheroids in defined, differentiation-promoting culture medium and investigated the effect of ethanol on early brain development. We have assessed the effects of ethanol on cortical development by monitoring cell death, morphological change, cellular proliferation, and the progenitor cell population with an emphasis on outer radial glia-like cells in the spheroids at cellular level. This proposed administrative supplement will allow the PI completing analysis of acquired RNA sequencing data in preparation of future manuscript. This administrative supplement will also provide the necessary resources allowing an orderly completion of the parent project.