Cell Transplantation and Analysis Core

项目总结（见说明）： 核心C（细胞移植和分析核心）的目的是为三个项目和核心B下进行的研究的细胞来源、分离和分析需求提供关键支持。核心C的具体服务目标是：目标1：从人类胚胎、胎儿、新生儿和成人组织标本中获取特定细胞类型和组织。项目科学家对皮肤、造血和间充质细胞等原代人体组织标本的要求将通过从胎儿、新生儿和成人标本中获得这些组织来满足。目标2：为项目科学家提供流式细胞仪分析和分选服务。将向项目科学家提供流式细胞仪分析和荧光激活细胞分选的专门知识和协助。目的3：检测免疫缺陷小鼠的畸胎瘤形成。诱导多能干细胞（IPS）的一个标志是它们在移植到免疫缺陷小鼠中时形成畸胎瘤的能力。将对3个项目生成的IPS细胞和分化细胞进行畸胎瘤形成能力测定，以测量多能性。目的4：检测IPS细胞诱导的候选造血干细胞（HSCs）的长期多系造血重建能力。将通过移植到免疫缺陷小鼠中，检测项目3中由IPS细胞产生的造血细胞重建红系、髓系和淋巴系至少12周的能力。本工作将在血液系统研究所的Marcus Muench博士的指导下进行，利用该机构已经建立的核心免疫学和细胞、组织和动物园核心。

PROJECT SUMMARY (See instructions): The purpose of Core C, the Cell Transplantation and Analysis Core, is to provide critical support for cell sourcing, isolation and analysis needs of research performed under the three Projects and Core B, The specific service aims of Core C are: Aim 1: To procure specific cell types and tissues from human embryonic, fetal, neonatal and adult tissue specimens. The requirements of project scientists for primary human tissue specimens such as skin, hematopoietic and mesenchymal cells will be met by obtaining these tissues from fetal, neonatal and adult specimens. Aim 2: To provide flow cytometric analysis and sorting services to project scientists. Expertise and assistance with flow cytometric analysis and fluorescence-activated cell sorting (FACS) will be provided to project scientists. Aim 3: To assay teratoma formation in immunodeficient mice. One hallmark of induced pluripotent stem (IPS) cells is their capacity to form teratomas when transplanted into immunodeficient mice. IPS cells and differentiated cells generated by the 3 projects will be assayed for teratoma forming ability to measure pluripotentiality. Aim 4: To assay long-term multilineage hematopoietic reconstitution of candidate hematopoietic stem cells (HSCs) generated from IPS cells. The capacity of hematopoietic cells created from IPS cells under Project 3 to reconstitute erythroid, myeloid and lymphoid lineages for at least 12 weeks will be tested by transplantation into immunodeficient mice. The work will be performed under the guidance of Dr. Marcus Muench at Blood systems Research Institute utilizing the Core Immunology and Cell, Tissue and Vivarium Cores already established at that institution.