PRIMATE PLURIPOTENT CELLS FOR AUTOLOGOUS TRANSPLANTATION

用于自体移植的灵长类多能细胞

• 批准号: 8173333
• 负责人: SHOUKHRAT M MITALIPOV
• 金额: $ 7.61万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173333
• 关键词: Adult; Autologous Transplantation; Cells; Chimera organism; Computer Retrieval of Information on Scientific Projects Database; Embryo; Fibroblasts; Funding; Grant; Institution; Macaca mulatta; Monkeys; Primates; Research; Research Personnel; Resources; Skin; Somatic Cell; Source; Testing; United States National Institutes of Health; Validation; embryonic stem cell; induced pluripotent stem cell; pluripotency; somatic cell nuclear transfer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this proposal is to investigate the potential of rhesus monkey pluripotent cells experimentally induced by reprogramming of adult somatic cells using two alternative approaches: somatic cell nuclear transfer (SCNT) and direct reprogramming. In this application, we will generate genetically identical SCNT-ES and iPS cells from adult monkey skin fibroblasts and compare them to each other and to ES cells produced from fertilized embryos using an array of extensive tests including the ability to generate chimeras, the ultimate validation of their pluripotency.

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本提案的目的是研究恒河猴多能细胞的潜力，实验诱导的成年体细胞重编程使用两种替代方法：体细胞核移植（SCNT）和直接重编程。在本申请中，我们将从成年猴皮肤成纤维细胞中产生遗传上相同的SCNT-ES和iPS细胞，并使用一系列广泛的测试将它们相互比较，并与从受精胚胎中产生的ES细胞进行比较，包括产生嵌合体的能力，最终验证其多能性。