PATHWAYS OF CONFORMATIONAL TRANSITIONS CRUCIAL FOR SIGNALING, ENZYME CATALYSIS

对信号传导、酶催化至关重要的构象转变途径

• 批准号: 8364312
• 负责人: DOROTHEE KERN
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364312
• 关键词: Award; Biological Process; Biomedical Research; Catalysis; Enzymes; Funding; Goals; Grant; High Performance Computing; Institutes; Investigation; Medical; Modeling; Molecular; National Center for Research Resources; Pathway interactions; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Signal Transduction; Signaling Protein; Source; System; United States National Institutes of Health; Work; conformational conversion; conformer; cost; protein protein interaction; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We propose a detailed investigation of the mechanism of conformational transition for three diverse proteins representing a signaling protein, an enzyme and a regulator through protein/protein interaction. Recent experiments conducted within our group on these three systems have shown that their ability of interconverting between different coexisting conformers is key to the accomplishment of their biological function. The goal of our investigations is to quantitatively model these conformational changes, and to understand at a molecular level the specific interactions most relevant in triggering and accompanying the interconversions. The proposed work is funded by the Dept of Energy (Award No. DE-FG02-05ER15699) and the Howard Hughes Medical Institute.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们提出了一个详细的调查机制的构象转变为三个不同的蛋白质代表的信号蛋白，酶和调节剂通过蛋白质/蛋白质相互作用。最近在我们小组对这三个系统进行的实验表明，它们在不同共存构象之间相互转换的能力是实现其生物学功能的关键。我们调查的目标是定量模拟这些构象变化，并在分子水平上了解触发和伴随的相互转化最相关的特定相互作用。拟议的工作是由能源部（奖励号DE-FG 02 - 05 ER 15699）和霍华德休斯医学研究所资助。