Triple Resonance Cryoprobe System for a 600 MHz NMR

要求为布兰迪斯大学现有的现代瓦里安Inova 600 MHz核磁共振光谱仪提供低温冷却，超高灵敏度三重共振，1H{13C/15N}， 5毫米探头和相关的闭式循环低温系统。冷冻探针技术已被证明为研究重要的生物大分子提供了显著的改进信号噪声比。600 MHz光谱仪目前大量用于高分辨率、高灵敏度的多维核磁共振实验，通常用于同位素标记（15N、13C、2H）溶液中的生物样品。低温探针/低温平台将显著提高仪器的灵敏度和通量。布兰迪斯大学的三名研究人员以及塔夫茨大学和波士顿大学的两名科学家是冷冻探针的最初主要用户。低温探针灵敏度的提高将使研究小组能够进行目前无法进行的实验

Funding is requested for a cryogenically-cooled, ultra high-sensitivity triple resonance, 1H{13C/15N}, 5 mm probe and associated closed-cycle cryogenic system for an existing modern Varian Inova 600 MHz NMR spectrometer at Brandeis University. The cryoprobe technology has been demonstrated to provide a significant improvement in signal-to noise ratios for studies of biologically important macromolecules. The 600 MHz spectrometer is currently heavily used for high-resolution, high-sensitivity multidimensional NMR experiments, typically with isotopically labeled (15N, 13C, 2H) biological samples in solution. The cryoprobe/cryoplatform will significantly increase the sensitivity and throughput of the instrument. Three investigators from Brandeis University along with two scientists from Tufts University and Boston University constitute the initial major users of the cryoprobe. The increase in sensitivity gained by the cryoprobe will allow the research groups to perform experiments that are currently not practical on the existing spectrometer. Technically demanding projects such as studies of the dynamics and structure of (i) enzymes during catalysis, (ii) shortly-lived macromolecular species and (iii) large proteins and protein/protein complexes for which only low concentrated samples can be reached will greatly benefit from the increase in signal-to noise ratio. Finally, the increased throughput of the system will accelerate the NIH supported research projects since NMR time is currently one limiting factor. The technical skills are available to maintain and operate the instrument at the NMR facility at Brandeis.