SONGBIRD NEUROGENOMICS

鸣鸟神经基因组学

• 批准号: 8364224
• 负责人: Erich D Jarvis
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364224
• 关键词: Behavioral; Biological; Biomedical Research; Brain; Communication; Complementary DNA; Databases; Funding; Genbank; Goals; Grant; High Performance Computing; Institutes; Japan; Length; Libraries; Messenger RNA; Molecular; National Center for Research Resources; Neurobiology; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Songbirds; Source; United States National Institutes of Health; Universities; abstracting; cDNA Arrays; cost; knowledge base; research study; vocal learning; zebra finch

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Keywords: cDNA, annotation, songbird, microarrays Abstract: The goal of this project is to generate a general purpose cDNA database resource of the songbird brain, for use in behavioral neurobiology experiments, particularly related to vocal learning. As of December 2002, the Genbank database lists on 72 cloned non-redundant zebra finch cDNAs, most of which are not full-length; this is certainly not enough to make evolutionary comparisons, cDNA microarrays, or other types of large scale neurogenomic comparisons. To narrow this molecular gap, we have formed a consortium of investigators from Duke University in the USA and RIKEN Institute in Japan, that has the following aims: 1) clone full-length cDNAs from a normalized zebra finch brain library that represents mRNAs expressed during commonly studied vocal communication states, 2) characterize these cDNAs by sequencing and forming a comprehensive knowledge base, and 3) use the cDNAs and knowledge base to generate and characterize non-reduntant microarrays for asking biological questions.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 保留字： cDNA，注释，鸣禽，微阵列 摘要： 本项目的目标是建立一个通用的cDNA数据库 鸣禽大脑的资源，用于行为神经生物学 实验，特别是与声乐学习有关的实验。截至2002年12月， Genbank数据库列出了72个克隆的非冗余斑胸草雀cDNA， 其中大部分都不是完整的长度;这当然不足以使 进化比较、cDNA微阵列或其他类型的大规模 神经基因组比较为了缩小这种分子间隙，我们形成了一个 来自美国杜克大学和RIKEN的研究人员组成的联盟 该研究所在日本，有以下目标：1）克隆全长 来自代表mRNA的标准化斑胸草雀脑文库的cDNA 在通常研究的语音通信状态期间表达，2） 通过测序来表征这些cDNA，并形成一个全面的 知识库，以及3）使用cDNA和知识库来产生和 表征非冗余微阵列以提出生物学问题。