SONGBIRD NEUROGENOMICS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 关键词： CDNA，注释，鸣禽，微阵列 摘要： 这个项目的目标是生成一个通用的cdna数据库 鸣禽脑资源，用于行为神经生物学 实验，特别是与声乐学习有关的实验。截至2002年12月， Genbank数据库列出了72个克隆的非冗余斑雀cDNA， 其中大部分不是全长的；这肯定不足以使 进化比较、cDNA微阵列或其他类型的大规模 神经基因组学比较。为了缩小这个分子鸿沟，我们形成了一个 由美国杜克大学和理工学院的调查人员组成的联盟 日本研究所，其目标如下：1)克隆全长 代表mRNAs的归一化斑马雀脑库中的cDNA 在通常研究的语音交流状态下表达，2) 通过对这些DNA进行测序并形成一个全面的 知识库，以及3)使用cDNA和知识库来生成和 描述用于提出生物学问题的非红色微阵列。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Keywords: cDNA, annotation, songbird, microarrays Abstract: The goal of this project is to generate a general purpose cDNA database resource of the songbird brain, for use in behavioral neurobiology experiments, particularly related to vocal learning. As of December 2002, the Genbank database lists on 72 cloned non-redundant zebra finch cDNAs, most of which are not full-length; this is certainly not enough to make evolutionary comparisons, cDNA microarrays, or other types of large scale neurogenomic comparisons. To narrow this molecular gap, we have formed a consortium of investigators from Duke University in the USA and RIKEN Institute in Japan, that has the following aims: 1) clone full-length cDNAs from a normalized zebra finch brain library that represents mRNAs expressed during commonly studied vocal communication states, 2) characterize these cDNAs by sequencing and forming a comprehensive knowledge base, and 3) use the cDNAs and knowledge base to generate and characterize non-reduntant microarrays for asking biological questions.