SONGBIRD NEUROGENOMICS

鸣鸟神经基因组学

• 批准号: 8364224
• 负责人: Erich D Jarvis
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364224
• 关键词: Behavioral; Biological; Biomedical Research; Brain; Communication; Complementary DNA; Databases; Funding; Genbank; Goals; Grant; High Performance Computing; Institutes; Japan; Length; Libraries; Messenger RNA; Molecular; National Center for Research Resources; Neurobiology; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Songbirds; Source; United States National Institutes of Health; Universities; abstracting; cDNA Arrays; cost; knowledge base; research study; vocal learning; zebra finch

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Keywords: cDNA, annotation, songbird, microarrays Abstract: The goal of this project is to generate a general purpose cDNA database resource of the songbird brain, for use in behavioral neurobiology experiments, particularly related to vocal learning. As of December 2002, the Genbank database lists on 72 cloned non-redundant zebra finch cDNAs, most of which are not full-length; this is certainly not enough to make evolutionary comparisons, cDNA microarrays, or other types of large scale neurogenomic comparisons. To narrow this molecular gap, we have formed a consortium of investigators from Duke University in the USA and RIKEN Institute in Japan, that has the following aims: 1) clone full-length cDNAs from a normalized zebra finch brain library that represents mRNAs expressed during commonly studied vocal communication states, 2) characterize these cDNAs by sequencing and forming a comprehensive knowledge base, and 3) use the cDNAs and knowledge base to generate and characterize non-reduntant microarrays for asking biological questions.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 关键字： cDNA，注释，鸣禽，微阵列 抽象的： 该项目的目的是生成通用cDNA数据库 鸣禽大脑的资源，用于行为神经生物学 实验，特别是与声带学习有关的实验。截至2002年12月， GenBank数据库列出了72个克隆的非冗余斑马Finch cDNA， 其中大多数不是全长；这当然还不够 进化比较，cDNA微阵列或其他类型的大型 神经基因组比较。为了缩小这个分子间隙，我们形成了一个 美国杜克大学和瑞肯的调查人员财团 日本学会的目标：1）克隆全长 代表mRNA的标准化斑马雀脑库的cDNA 在经常研究的人声交流状态下表达2） 通过测序和形成全面来表征这些CDNA 知识基础，以及3）使用cDNA和知识库生成和 特征非退缩微阵列提出生物学问题。