STRUCTURAL DETERMINANTS OF FLICKERING IN K+ CHANNELS

K 通道闪烁的结构决定因素

• 批准号: 8364329
• 负责人: BENOIT ROUX
• 金额: $ 0.11万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364329
• 关键词: Biomedical Research; Data; Funding; Goals; Grant; High Performance Computing; Life; Molecular Conformation; National Center for Research Resources; Potassium Channel; Principal Investigator; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; base; cost; molecular dynamics; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to use classical trajectories of the KcsA channel to elucidate the structural basis of the short-lived nonconductive conformational states of the selectivity filter that are associated with the rapid flickering observed in single-channel recordings. Based on available experimental data, flickering is expected to arise naturally during multi-?s molecular dynamics trajectories of the open E71Q mutant of the KcsA channel. The trajectories will be used to generate new experimentally testable hypotheses regarding the transient conformations of the selectivity filter.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 我们的目标是使用KCSA通道的经典轨迹来阐明与单渠道记录中观察到的快速闪烁相关的选择性滤波器的短寿命非导导构象状态的结构基础。基于可用的实验数据，预计在KCSA通道的开放E71Q突变体的多个分子动力学轨迹中，自然会出现闪烁。这些轨迹将用于生成有关选择性过滤器的瞬态构象的新的实验测试假设。