SPECKLE CORRELATION MICROSCOPY FOR INVERTING TURBIDITY

用于反演浊度的散斑相关显微镜

• 批准号: 8364162
• 负责人: YOHAN CHOI
• 金额: $ 0.74万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364162
• 关键词: Biological; Biomedical Research; Complex; Elements; Event; Exhibits; Funding; Grant; Heterogeneity; Image; Lasers; Measures; Microscope; Microscopy; National Center for Research Resources; Optics; Principal Investigator; Refractive Indices; Research; Research Infrastructure; Resolution; Resources; Sampling; Source; Spatial Distribution; Specimen; Surface; Thick; Tissues; United States National Institutes of Health; Work; cost; detector; light scattering; nanoparticle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Optically opaque media such as thick biological tissues possess complex spatial distributions of refractive index. Multiple light scattering events due to this refractive index heterogeneity undermine the working principle of optical microscopy, which is to focus waves originating from an object to distinct points of the detector plane. Consequently, spatial resolution is significantly degraded and sampling depth is limited to the shallow surface of a specimen. In this project, we will develop an interferometric microscope for converting a turbid medium into a deterministic optical element by quantitatively measuring and then deterministically eliminating the effects of refractive index heterogeneity. High-resolution imaging of objects hidden behind highly turbid media, such as a layer of ZnO nano-particles exhibiting more than 10 scattering events on average, will be performed.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 光学不透明介质，如厚的生物组织，具有复杂的折射率空间分布。由于这种折射率不均匀性而导致的多个光散射事件破坏了光学显微镜的工作原理，光学显微镜的工作原理是将源自物体的波聚焦到检测器平面的不同点。因此，空间分辨率显着降低，采样深度仅限于样品的浅表面。在本计画中，我们将开发一种干涉显微镜，借由定量测量，然后确定性地消除折射率不均匀性的影响，将混浊介质转换成确定性的光学元件。将对隐藏在高度混浊介质后面的物体进行高分辨率成像，例如平均表现出超过10次散射事件的ZnO纳米颗粒层。