Imaging Resource

影像资源

• 批准号: 7669779
• 负责人: TOMAS KIRCHHAUSEN
• 金额: $ 47.33万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-25 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7669779
• 关键词: Algorithms; Arts; Bacteria; Cell physiology; Cells; Communities; Computer software; Containment; Core Facility; Data Analyses; Data Collection; Detection; Development; Elements; Equipment; Event; Fluorescence Microscopy; Funding; Human Resources; Image; Infectious Agent; Label; Laboratories; Life; Lipids; Listeria; Mammalian Cell; Membrane; Membrane Protein Traffic; Microscope; Molecular; New England; Optics; Pathology; Process; Proteins; Reovirus; Research Personnel; Resolution; Resources; Time; Toxin; Training; Tubular formation; Update; Vesicular stomatitis Indiana virus; Viral; Virus; Work; antimicrobial; base; biodefense; cellular imaging; data acquisition; flexibility; instrumentation; interest; light microscopy; meetings; optical imaging; pathogen; protein transport; single molecule; tool; trafficking

Recent developments in fluorescence microscopy geared for single-molecule detection, optical sectioning and three-dimensional data acquisition, used in combination with object-identification algorithms, now allow sufficient temporal and spatial resolution to visualize the sequential recruitment and intracellular traffic of proteins, lipids, and pathogens in living cells including viruses and bacteria. The Kirchhausen laboratory has a longstanding interest in understanding the mechanisms underlying entry of toxins, viruses and bacteria into cells, and how these processes relate to the organization and intracellular traffic of vesiculo-tubular membrane carriers. As part of this effort, we have devoted substantial efforts to developing an imaging suite containing state-of-the-art microscopes and supporting software suited for data collection and analysis with high spatial and temporal resolution. It is also a priority to maintain an imaging suite that is 100% accessible to all users, so that after initial training, any investigator can perform their work with total flexibility and independence. In this proposal we outline plans for a NERCE Imaging Core facility that will provide access to contemporary tools and expertise for live-cell and single molecule imaging aimed towards, but not limited to, quantitative descriptions of mechanisms of invasion for bacterial and viral pathogens into mammalian cells, of viral replication, and of molecular aspects related to toxin entry into cells.

荧光显微镜在单分子检测、光学切片 与物体识别算法结合使用的三维数据采集现在允许 足够的时间和空间分辨率，以可视化的顺序招聘和细胞内交通 活细胞中的蛋白质、脂质和病原体，包括病毒和细菌。基尔希豪森实验室 长期以来，人们一直对了解毒素、病毒和细菌进入人体的机制感兴趣， 细胞，以及这些过程如何与泡状小管的组织和细胞内交通 膜载体作为这项工作的一部分，我们投入了大量的精力来开发一个成像套件 包含最先进的显微镜和支持软件，适用于数据收集和分析， 高空间和时间分辨率。维护一个100%可访问的映像套件也是一个优先事项 向所有用户开放，这样，经过初步培训后，任何调查员都可以完全灵活地开展工作， 独立 在本提案中，我们概述了NERCE成像核心设施的计划，该设施将提供当代 活细胞和单分子成像的工具和专业知识，旨在但不限于定量 描述了细菌和病毒病原体侵入哺乳动物细胞的机制， 复制，以及与毒素进入细胞相关的分子方面。