Bio-optical and Bio-electronic Materials for Real-time Analyte Detection

用于实时分析物检测的生物光学和生物电子材料

• 批准号: 7591704
• 负责人: Kevin W Plaxco
• 金额: $ 23.86万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591704
• 关键词: Address; Autoimmune Diseases; Binding; Blood; Blood specimen; Chemistry; Complex; Coupled; Coupling; Detection; Development; Devices; Diagnosis; Diagnostic; Diagnostic Sensitivity; Diagnostic tests; Disease; Electrochemistry; Electrodes; Electronics; Elements; Epitopes; Fluorescence; Goals; Gold; HIV; HIV Antibodies; HIV Infections; Hand; Head; Health; Health Status; Healthcare; Hour; Label; Laboratories; Length; Measurement; Medical; Methods; Molecular; Molecular Target; Monitor; Optical reporter; Optics; Output; Peptides; Pharmaceutical Preparations; Procedures; Proteins; Proxy; Reporter; Research; Resources; Safety; Sampling; Sensitivity and Specificity; Serum; Signal Transduction; Solutions; Speed; Surface; Techniques; Technology; Testing; Time; Treatment Efficacy; Validation; Whole Blood; analog; base; clinical application; clinical material; clinically relevant; compliance behavior; improved; infectious disease treatment; internal control; meetings; monolayer; peptide E (adrenal medulla); point of care; point-of-care diagnostics; polypeptide; programs; public health relevance; response; sensor

DESCRIPTION (provided by applicant): Current methods for the detection of diagnostic proteins entail time- and resource-intensive immunochemical techniques that, even under ideal circumstances, require an hour or more to return an answer to the clinician's hands. This sluggish response fits poorly into the timeframe of modern healthcare, hindering patient compliance and, in some circumstances, the efficiency and safety with which drugs and procedures are administered. (Consider: a diagnostic test that returns an answer in less than 15 minutes differs qualitatively from a test that requires 30 minutes because the latter requires a second interaction with the clinician.) Here we propose the development of a rapid, point-of-care method for the simultaneous detection of multiple diagnostic proteins. Our approach, which utilizes electrochemistry to monitor the binding-induced folding of polypeptide- or protein-based recognition elements, will be rapid, specific, convenient and, critically, selective enough to employ directly in blood serum and -we propose- whole blood. And while our initial development efforts will focus on the detection of proteins diagnostic of HIV infection (such as anti-HIV antibodies), the approach will be general enough to be of use in the detection of a wide range of clinically relevant markers. The development of rapid, parallelizable point-of-care diagnostics could significantly impact the safety, compliance and efficacy of therapies and medical procedures ranging from the detection of infectious diseases, the treatment of autoimmune diseases, and the routine monitoring of health status. PUBLIC HEALTH RELEVANCE Here we proposed the development of a reagentless, electrochemical platform for the simultaneous detection of multiple proteins diagnostic of disease. The proposed technology will be rapid, specific, and selective enough to employ directly at the point of care, thus significantly improving the speed with which molecular diagnostics can be performed and their results acted upon. This, in turn, will improve the safety, compliance and efficacy of therapies and procedures ranging from the detection of infectious diseases, the treatment of autoimmune diseases, and the routine monitoring of health status.

描述（由申请人提供）：目前用于检测诊断蛋白的方法需要时间和资源密集型免疫化学技术，即使在理想情况下，也需要一个小时或更长时间才能将答案返回给临床医生。这种缓慢的反应与现代医疗保健的时间框架不相适应，阻碍了患者的依从性，并且在某些情况下，还影响了药物和程序的管理效率和安全性。（考虑：在少于15分钟内返回答案的诊断测试与需要30分钟的测试在性质上不同，因为后者需要与临床医生的第二次交互。在这里，我们提出了一种快速的，即时的护理方法，用于同时检测多种诊断蛋白质的发展。我们的方法，利用电化学监测多肽或蛋白质为基础的识别元素的结合诱导的折叠，将是快速，特异性，方便，关键是，选择性足以直接在血清和-我们建议-全血。虽然我们最初的开发工作将集中在检测诊断HIV感染的蛋白质（如抗HIV抗体）上，但该方法将具有足够的通用性，可用于检测广泛的临床相关标志物。快速、可并行的护理点诊断的发展可能会显著影响治疗和医疗程序的安全性、依从性和有效性，包括传染病的检测、自身免疫性疾病的治疗和健康状况的常规监测。 公共卫生相关性在这里，我们提出了一种无试剂的电化学平台的发展，用于同时检测多种蛋白质的疾病诊断。所提出的技术将是快速的，特异性的和选择性的，足以直接在护理点使用，从而显着提高分子诊断的速度，并对其结果采取行动。反过来，这将提高治疗和程序的安全性、依从性和有效性，包括传染病的检测、自身免疫性疾病的治疗和健康状况的常规监测。