Genome Engineering of Cryptococcus Neoformans using CRISPR-Cas System

使用 CRISPR-Cas 系统对新型隐球菌进行基因组工程

• 批准号: 9197632
• 负责人: PING WANG
• 金额: $ 7.3万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9197632
• 关键词: Animals; Antifungal Agents; Antifungal Therapy; Clustered Regularly Interspaced Short Palindromic Repeats; Cryptococcus; Cryptococcus neoformans; Custom; Cyclosporine; Development; FK506; Gene Mutation; Genes; Genome engineering; Genomic approach; Genomics; Goals; Growth; Guide RNA; Human; Immune; Immunophilins; Individual; Life; Melanins; Meningoencephalitis; Modification; Molecular; Molecular Genetics; Organism; Pathogenesis; Pathogenicity; Pigments; Plants; Plasmid Cloning Vector; Polysaccharides; Production; Proteins; Resistance; Sirolimus; System; Technology; Testing; Virulence; Virulence Factors; Yeasts; capsule; design and construction; endonuclease; expression vector; fungus; genetic technology; genome editing; immunological status; innovation; novel; pathogen; public health relevance; tool; trait

 DESCRIPTION (provided by applicant): Cryptococcus neoformans is an opportunistic human pathogen that causes potentially fatal meningoencephalitis in individuals with immune deficiency. Virulence of C. neoformans is a multi-faceted trait with the production of the melanin pigment, a polysaccharide capsule, and secreted proteins contributing to its virulence. The recent introduction of molecular and genetic technologies have allowed us to begin to understand the molecular basis of fungal virulence, yet precision gene alterations and efficient multiplex genome editing that allows systematic examination of virulence mechanisms remain limited due to the lack of advanced approaches. We propose to develop a CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) system for precise gene specific modification and efficient multiplex genome editing in C. neoformans. The successful development of this innovative genomic approach will provide a revolutionary tool in our studying of virulence mechanisms in C. neoformans.



项目成果

MoMip11, a MoRgs7-interacting protein, functions as a scaffolding protein to regulate cAMP signaling and pathogenicity in the rice blast fungus Magnaporthe oryzae.

MoMip11 是一种 MoRgs7 相互作用蛋白，在稻瘟病菌 Magnaporthe oryzae 中作为支架蛋白调节 cAMP 信号传导和致病性

• DOI: 10.1111/1462-2920.14102
• 发表时间: 2018-09
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Yin Z;Zhang X;Wang J;Yang L;Feng W;Chen C;Gao C;Zhang H;Zheng X;Wang P;Zhang Z
• 通讯作者: Zhang Z

Comparative proteomic analysis of Gib2 validating its adaptor function in Cryptococcus neoformans.

• DOI: 10.1371/journal.pone.0180243
• 发表时间: 2017
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Bruni GO;Battle B;Kelly B;Zhang Z;Wang P
• 通讯作者: Wang P

MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.

• DOI: 10.1371/journal.pgen.1006814
• 发表时间: 2017-05
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Li L;Chen X;Zhang S;Yang J;Chen D;Liu M;Zhang H;Zheng X;Wang P;Peng Y;Zhang Z
• 通讯作者: Zhang Z

MoYvh1 subverts rice defense through functions of ribosomal protein MoMrt4 in Magnaporthe oryzae.

MoYvh1 通过核糖体蛋白 MoMrt4 的功能破坏稻瘟病菌的防御

• DOI: 10.1371/journal.ppat.1007016
• 发表时间: 2018-04
• 期刊: PLoS pathogens
• 影响因子: 6.7
• 作者: Liu X;Yang J;Qian B;Cai Y;Zou X;Zhang H;Zheng X;Wang P;Zhang Z
• 通讯作者: Zhang Z

MoPpe1 partners with MoSap1 to mediate TOR and cell wall integrity signalling in growth and pathogenicity of the rice blast fungus Magnaporthe oryzae.

MoPpe1 与 MoSap1 合作，介导稻瘟病菌 Magnaporthe oryzae 生长和致病性中的 TOR 和细胞壁完整性信号传导

• DOI: 10.1111/1462-2920.14421
• 发表时间: 2018-11
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Qian B;Liu X;Jia J;Cai Y;Chen C;Zhang H;Zheng X;Wang P;Zhang Z
• 通讯作者: Zhang Z