MicroScale Thermophoresis Instrument for Life Sciences

项目总结/摘要 波士顿大学化学系寻求NIH的支持，以获得一个微型热泳 (MST)Charles River Campus（CRC）这一工具将使调查人员能够在 CRC将推进他们在生命过程（化学，生物化学和生物学）方面的研究，并允许他们 研究进入新的领域，将丰富学生和博士后培训。仪器的 能力将加强积极的研究项目，涉及定量蛋白质和蛋白质， 配体相互作用以及鉴定多亚基中发生的蛋白质构象变化 蛋白质复合物作为氧化还原状态或变构活化的函数。所要求的文书对以下方面至关重要： 十一个主要用户（艾伦教授、贝弗特教授、比勒教授、布朗教授、卡拉多纳教授、埃利奥特教授， Harris，Ho，Perlstein，Porco和Whitty）。MST仪器将在BU的生命扩展中发挥关键作用 生物物理研究中的科学。大学的承诺体现在其对 支持多用户研究仪器的基础设施，并为全职技术人员提供工资 支持. 2006年，BU完成了5，500平方米的建设-英尺化学仪表中心（CIC）， 在设计时，为生物物理仪器配备了专门的空间和支助人员。定量 化学生物学领域，依赖于生物物理表征方法来了解相互作用 分子之间的联系继续在部门内增长，包括最近七次招聘中的五次。 化学和生物学。所要求的仪器将提高我们在化学品审查委员会方面的生物物理能力， 教师和学生当前和不断发展的研究需求。该中心主任李诺曼博士将于 管理所请求的仪器的使用和财务，生物物理学家杰弗里·培根博士 仪器专家将操作、维护并提供所需仪器的用户培训。的 中投公司对仪器的使用、财务监督和科学管理有一个示范性的管理方案， 监督机制包括一个由用户和非用户代表组成的九人咨询委员会。的 用户实验室的研究生和博士后研究员将广泛使用该仪器 作为他们研究的一部分。对青年科学家的这种培训将促进他们的研究目标，同时提供 他们有额外的技术技能，在他们未来的职业生涯取得成功。

Project Summary/Abstract The Boston University Department of Chemistry seeks NIH support to acquire a MicroScale Thermophoresis (MST) instrument for the Charles River Campus (CRC). This instrument would enable investigators on the CRC to advance their research in life processes (chemistry, biochemistry and biology) and allow their investigations to move into new areas that would enrich student and postdoctoral training. The instrument's capabilities would enhance active research projects involving the quantification of protein-protein and protein- ligand interactions as well as the identification of protein conformation changes that occur in multi-subunit protein complexes as a function of redox state or allosteric activation. The requested instrument is critical to the research programs of eleven Major Users (Professors Allen, Beffert, Beeler, Brown, Caradonna, Elliott, Harris, Ho, Perlstein, Porco and Whitty). The MST instrument will play a pivotal role in BU's expansion of its life science in biophysical research. The University's commitment is demonstrated by its investment in the infrastructure for supporting multi-user research instruments and by its provision of salary for full time technical support. In 2006, BU completed construction of the 5,500 sq.-ft. Chemical Instrumentation Center (CIC), which was designed with specific space and support staff designated for biophysical instrumentation. The quantitative chemical biology areas that rely on biophysical characterization methods to understand the interactions between molecules continue to grow within the departments, including five of the seven most-recent hires in chemistry and in biology. The requested instrument will enhance our biophysical capability on CRC to meet the current and evolving research needs of the faculty and students. The CIC's Director, Dr. Norman Lee, will manage the usage and finances of the requested instrument and Dr. Jeffrey Bacon, a biophysical instrumentation specialist will operate, maintain and provide user training on the requested instrument. The CIC has a demonstrated management plan for the instrument's usage, financial supervision and scientific oversight, including a nine-member Advisory Committee with representatives from users and non-users. The graduate students and postdoctoral fellows in the laboratories of the users will use this instrument extensively as part of their research. This training of young scientists will facilitate their research objectives, while providing them with additional technical skills for succeeding in their future professional careers.