MicroScale Thermophoresis Instrument for Life Sciences

生命科学微型热泳仪

• 批准号: 9274743
• 负责人: Norman CY Lee
• 金额: $ 13.67万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2018-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9274743
• 关键词: Advisory Committees; Area; Biochemistry; Biological Sciences; Biology; Biophysics; Boston; Chemicals; Chemistry; Faculty; Future; Investigation; Investments; Laboratories; Life; Ligands; Methods; Oxidation-Reduction; Play; Postdoctoral Fellow; Process; Protein Complex Subunit; Protein Conformation; Proteins; Research; Research Infrastructure; Research Personnel; Research Project Grants; Rivers; Role; Scientist; Specialist; Students; Supervision; Technical Expertise; Time; Training; United States National Institutes of Health; Universities; Wages; biophysical properties; career; design; graduate student; instrument; instrumentation; member; post-doctoral training; professor; programs; student training

Project Summary/Abstract The Boston University Department of Chemistry seeks NIH support to acquire a MicroScale Thermophoresis (MST) instrument for the Charles River Campus (CRC). This instrument would enable investigators on the CRC to advance their research in life processes (chemistry, biochemistry and biology) and allow their investigations to move into new areas that would enrich student and postdoctoral training. The instrument's capabilities would enhance active research projects involving the quantification of protein-protein and protein- ligand interactions as well as the identification of protein conformation changes that occur in multi-subunit protein complexes as a function of redox state or allosteric activation. The requested instrument is critical to the research programs of eleven Major Users (Professors Allen, Beffert, Beeler, Brown, Caradonna, Elliott, Harris, Ho, Perlstein, Porco and Whitty). The MST instrument will play a pivotal role in BU's expansion of its life science in biophysical research. The University's commitment is demonstrated by its investment in the infrastructure for supporting multi-user research instruments and by its provision of salary for full time technical support. In 2006, BU completed construction of the 5,500 sq.-ft. Chemical Instrumentation Center (CIC), which was designed with specific space and support staff designated for biophysical instrumentation. The quantitative chemical biology areas that rely on biophysical characterization methods to understand the interactions between molecules continue to grow within the departments, including five of the seven most-recent hires in chemistry and in biology. The requested instrument will enhance our biophysical capability on CRC to meet the current and evolving research needs of the faculty and students. The CIC's Director, Dr. Norman Lee, will manage the usage and finances of the requested instrument and Dr. Jeffrey Bacon, a biophysical instrumentation specialist will operate, maintain and provide user training on the requested instrument. The CIC has a demonstrated management plan for the instrument's usage, financial supervision and scientific oversight, including a nine-member Advisory Committee with representatives from users and non-users. The graduate students and postdoctoral fellows in the laboratories of the users will use this instrument extensively as part of their research. This training of young scientists will facilitate their research objectives, while providing them with additional technical skills for succeeding in their future professional careers.

项目摘要/摘要 波士顿大学化学系寻求美国国立卫生研究院的支持，以获得一种微型热电泳法 (MST)查尔斯河校区(CRC)仪器。这一工具将使调查人员能够 促进他们在生命过程(化学、生物化学和生物学)方面的研究，并允许他们的 调查将进入新的领域，以丰富学生和博士后培训。该仪器的 能力将加强积极的研究项目，涉及量化蛋白质-蛋白质和蛋白质- 配体相互作用以及多亚基中蛋白质构象变化的鉴定 蛋白质复合体作为氧化还原状态或变构激活的函数。所请求的仪器对于 11个主要用户(Allen，Beffert，Beeler，Brown，Caradonna，Elliott， Harris，Ho，Perlstein，Porco和Whitty)。MST仪器将在BU的寿命延长中发挥关键作用 生物物理研究中的科学。该大学的承诺体现在它对 支持多用户研究仪器的基础设施，并提供全职技术人员的工资 支持。2006年，BU完成了5500平方英尺的建设。化学仪器中心(CIC) 设计了为生物物理仪器指定的特定空间和支助人员。量化的 依赖生物物理表征方法来了解相互作用的化学生物学领域 部门内部的分子之间继续增长，包括最近招聘的七名员工中的五名 化学和生物学。所要求的仪器将加强我们在CRC上的生物物理能力，以满足 教师和学生当前和不断发展的研究需求。CIC主任诺曼·李博士将 管理所需仪器和生物物理学家杰弗里·培根博士的使用和财务 仪器专家将操作、维护并为用户提供所需仪器的培训。这个 中投公司对该工具的使用、财务监管和科学管理有示范的管理方案 监督，包括一个由9名成员组成的咨询委员会，其中包括用户和非用户的代表。这个 用户实验室的研究生和博士后将广泛使用该仪器 作为他们研究的一部分。这种对青年科学家的培训将促进他们的研究目标，同时提供 他们拥有额外的技术技能，以便在未来的职业生涯中取得成功。