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TECHNOLOGY NMR SPECTROSCOPY

核磁共振波谱技术

基本信息

批准号：
9346058
负责人：
K Sreekumaran Nair
金额：
$ 25.43万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9346058
关键词：
Aging Animals Area Biochemical Pathway Bioinformatics Biological Markers Blood specimen Body Fluids Brain Carbon Cardiovascular system Cerebrospinal Fluid Clinic Clinical Core Facility Cost Effectiveness Analysis Data Data Analyses Data Display Deposition Diabetes Mellitus Diagnostic Ensure Exercise Goals Heart Human Image Analysis Institution Instruction Kidney Label Liver Malignant Neoplasms Mass Spectrum Analysis Measurement Metabolic Methods Monitor Muscle NMR Spectroscopy Neurodegenerative Disorders Nuclear Magnetic Resonance Organ Pathway interactions Patient Monitoring Plasma Procedures Process Protocols documentation Quality Control Regenerative Medicine Research Research Personnel Resolution Resources Sampling Series Services Specialized Center Spectrum Analysis Standardization Stress Technology Testing Tissue Extracts Tissues Tracer Training Translating United States National Institutes of Health Urine Whole Blood Work advanced disease base biomarker panel data acquisition data sharing disease diagnosis drug development experience high standard high throughput screening human disease human tissue improved in vivo individualized medicine instrument instrumentation metabolomics metabolomics resource novel outcome forecast outreach personalized medicine programs spectroscopic imaging stable isotope tool treatment strategy

项目摘要

As an integral part of Mayo Clinic Comprehensive Metabolomics Resource Core (MCCMRC) the NMR Metabolomics core provides targeted analysis of metabolomic profiles using Nuclear Magnetic Resonance (NMR) Spectroscopy. In addition to establishing standard NMR high-throughput 1H and 31P NMR metabolomics analyses, the core will implement, its unique methods of 18O labeling and 18O-assisted 31P NMR spectroscopy for studies of phosphometabolite dynamics and 13C NMR for carbon isotopomer exchange in metabolic networks, a procedure that is integrated in Mass Spectrometry core. This integrated approach of qualitative and quantitative analysis of tissue extracts (1H and 31P NMR), stable isotope tracing (13C and 18O-assisted 31P NMR spectroscopy) and in vivo spectroscopy (1H, 31P volume selective NMR spectroscopy, NMR spectroscopic imaging) will include the analysis of human and animal samples (e.g., whole blood, plasma, cerebrospinal fluid, urine, heart, liver, brain, kidney) and in vivo intact organs (brain, heart, kidney, muscle). In addition, we will implement protocols for stress metabolomic testing before and after exercise. The NMR Metabolomics core is supported by Analytical NMR Facility that has 5 state-of-theart 300 MHz, 500 MHz, 600 MHz, 700 MHz and wide bore 700 MHz NMR spectrometers and is staffed with experienced NMR spectroscopists. We propose the acquisition of a high sensitivity 600 MHz BB cryoprobe, high-throughput sample handler and additional technical staff to expand the core capacity and analytical capabilities at least threefold for 1H and 4-9 fold for 31P and 13C NMR (from -25 to 100 samples/day) and to offer services and to a larger number of investigators inside and outside Mayo Clinic. The core will implement standardized metabolomic protocols and methods of NMR data acquisition, processing, metabolite identification, bioinformatic pathway and flux analysis and interpretation. The core will develop appropriate workflow that will ensure cost-effective analysis of series of samples, and quality control. The core staff will work with the MCCMRC Management to implement consistent formats to deposit raw, processed and analyzed data according to the data sharing plan. The NMR Metabolomic core currently assists over 20 NIH supported investigators in cardiovascular, aging, diabetes, cancer and neurodegenerative disease, and regenerative and individualized medicine areas. We plan to make the program self-sustaining in five years. Thus the overall goal of this application is to consolidate and expand the NMR Metabolomic core facility and services it provides and to offer our expertise to other national institutions serving as a regional hub to advance stable isotope and NMR based translational and clinical metabolomics.

作为梅奥诊所综合代谢组学资源核心（MCCMRC）的组成部分