Coordinating cellular events during spore development

项目摘要/摘要： 这个项目将研究配子发生过程中细胞事件的协调。在.期间 配子的形成，细胞必须协调发生在减数分裂和 配子分化。减数分裂是指当细胞经历 从二倍体细胞产生单倍体细胞核所必需的减数分裂。配子形成 需要对细胞形态进行广泛的改变，这些形态改变必须是 随着细胞的分化，与减数分裂相协调。 在萌芽酵母中，配子发生通过以下过程发生： 产孢子。在产孢子过程中，减数分裂涉及到孢子的形成。 膜，它生长以包围并最终包围减数分裂核；最终， 孢子膜成为新形成细胞的质膜。我们的长期合作 目的是了解孢子膜的发育，以获得基础知识 关于复杂的细胞活动是如何协调的。因为孢子膜是至关重要的 对于真菌病原体的生命周期，这些研究也可能提供对真菌特有的洞察 可以有针对性地对抗导致人类疾病的病原体的机制。 我们以前的研究表明，及时关闭孢子膜需要STE20 由SPS1编码的GC家族激酶，与SPO77共同作用，并与AMA1(AMA1)并行 编码后期促进复合体的减数分裂激活剂)。我们发现SPS1的作用是 通过PI(4，5)结合蛋白Ssp1调节孢子膜关闭，影响 Ssp1的稳定性和磷酸化。该方案中的实验扩展了我们之前的工作， 重点研究孢子膜闭合是如何协调的。AIM 1检查已知的 在孢子膜关闭中的作用，并分析它们与减数分裂调节因子的关系。 目标2试图更广泛地研究PSM闭合和细胞之间的关系 在减数分裂退出过程中发生的事件。

Project Summary/Abstract: This project will examine the coordination of cellular events during gametogenesis. During gamete formation, cells must coordinate the complex events that occur during meiosis and gamete differentiation. Meiosis involves the cellular events that occur as cells undergo the reductional divisions necessary to create haploid nuclei from a diploid cell. Gamete formation requires extensive changes to cell morphology, and these morphological changes must be coordinated with meiosis as cells differentiate. In the budding yeast Saccharomyces cerevisiae, gametogenesis occurs through the process of sporulation. During sporulation, meiotic cytokinesis involves the formation of the prospore membrane, which grows to surround and eventually enclose the meiotic nucleus; eventually, the prospore membrane becomes the plasma membrane of the newly formed cells. Our long-term goal is to understand prospore membrane development in order to gain fundamental knowledge about how complex cellular events are coordinated. Because the prospore membrane is critical for the life cycle of fungal pathogens, these studies may also provide insights into fungal-specific mechanisms that can be targeted to combat pathogens that cause human disease. Our previous studies show that timely closure of the prospore membrane requires the STE20 GC family kinase encoded by SPS1, which acts with SPO77 and in parallel to AMA1 (which encodes the meiotic activator of the anaphase promoting complex). We found that SPS1 acts to regulate prospore membrane closure through the PI(4,5) binding protein Ssp1, affecting the stability and phosphorylation of Ssp1. Experiments in this proposal extend our previous work, focusing on how prospore membrane closure is coordinated. Aim 1 examines genes known to act in prospore membrane closure and analyzes their relationship with regulators of meiosis. Aim 2 seeks to more broadly examine the relationship between PSM closure and the cellular events occurring during meiotic exit.