WU-SN-TMC Biospecimen Core

WU-SN-TMC 生物样本核心

• 批准号: 10376525
• 负责人: Ryan C Fields
• 金额: $ 19.2万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10376525
• 关键词: WU; SN; TMC; Biospecimen; Core

Biospecimen Core Summary/Abstract Senescence is a complex phenotype that likely consists of many states that have been implicated in human health and disease. We aim to create the Washington University Senescence Tissue Mapping Center (WU- SN-TMC), which will leverage our expansive infrastructure to generate multi-omics and imaging data to build high resolution, multi-dimensional atlases of cellular senescence across tissue types and lifespan that can be used to enable basic and translational senescence research. Our WU-SN-TMC will develop cellular senescence atlases using 500 human samples for four essential tissue types: bone marrow, breast, colon, and liver. To tackle such an ambitious goal, the WU-SN-TMC Biospecimen Core (WU-SN-TMC BC) has been designed to facilitate the collection of human tissues across a wide range of ages. In addition, the WU-SN- TMC BC will collect multiple tissues from individuals to allow us to compare senescent numbers and states across tissues from a single individual. The WU-SN-TMC BC will accomplish this goal by using sample acquisition pipelines that are divided into four tissue prospective collection protocols (CPs) that leverage WU clinical expertise and patient volume to collect relevant biospecimens. These CPs include: 1) CP1, which will collect bone marrow from normal donors without chronic disease across ages; 2) CP2, which includes normal breast, bone marrow and skin from patients undergoing cosmetic breast surgery across ages; 3) CP3, which includes patients undergoing endoscopy who will provide normal stomach, small bowel, and colon across ages; and 4) CP4 includes patients undergoing laparoscopic upper gastrointestinal surgery that will provide normal bone marrow, liver, and skin, again across ages. While our focus is on senescence during human aging, some patients in CP2 and CP4 will have received prior chemotherapy for cancers in unrelated tissues (i.e. biospecimens will be from unaffected, cancer-free tissues). This cohort will provide the opportunity to compare age-matched controls +/- chemotherapy and allow perturbations tested in mice in the Biological Analysis Core (BAC) to be mapped back to human tissue for relevance, further enriching the bone marrow, breast, colon, and liver atlases we will produce. The existing infrastructure at WU, our patient volume and the expertise of our team will ensure the success of the WU-SN-TMC BC.

Biosecimen核心摘要/摘要 衰老是一种复杂的表型，可能由许多与人类有关的状态组成 健康和疾病。我们的目标是创建华盛顿大学衰老组织测绘中心(吴- SN-TMC)，它将利用我们庞大的基础设施来生成多组学和成像数据，以建立 跨组织类型和寿命的高分辨率多维细胞衰老图谱 用于实现基础和转译衰老研究。我们的WU-SN-TMC将发展成细胞化 使用500份人体样本绘制了四种基本组织类型的衰老图谱：骨髓、乳房、结肠、 还有肝脏。为了实现这样一个雄心勃勃的目标，WU-SN-TMC生物样品核心(WU-SN-TMC BC)已经 旨在方便收集不同年龄段的人体组织。此外，WU-SN- TMC BC将收集个体的多个组织，使我们能够比较衰老的数量和状态 从单个个体的组织中分离出来。WU-SN-TMC BC将通过使用SAMPLE实现这一目标 采集管道分为四个利用WU的组织前瞻性采集协议(CP) 临床专业知识和病人数量，以收集相关的生物标本。这些CP包括：1)CP1，它将 采集正常捐献者的骨髓，不同年龄的人都没有慢性病；2)CP2，包括正常 乳房、骨髓和皮肤来自不同年龄段的整形乳房手术患者；3)CP3，它 包括接受内窥镜检查的患者，他们将提供正常的胃、小肠和结肠横跨 年龄；4)CP4包括接受腹腔镜上胃肠道手术的患者，该手术将提供 正常的骨髓、肝脏和皮肤，同样是不同年代的。虽然我们关注的是人类的衰老 随着年龄的增长，CP2和CP4中的一些患者将因无关组织的癌症而事先接受过化疗 (即生物样品将来自未受影响的、无癌症的组织)。这批人将提供机会 比较年龄匹配的对照组+/-化疗并允许在小鼠身上进行生物扰动测试 分析核心(BAC)将被映射回人体组织进行相关性，进一步丰富骨髓， 我们将制作乳房、结肠和肝脏图谱。吴医院现有的基础设施、我们的病人数量和 我们团队的专业知识将确保WU-SN-TMC BC的成功。