Core B: Biospecimen Core

核心 B：生物样本核心

• 批准号: 10708577
• 负责人: Ryan C Fields
• 金额: $ 24.48万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-28 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10708577
• 关键词: Adenocarcinoma Cell; Aftercare; Attention; Biological Models; Biological Specimen Banks; Biology; Biopsy; Blood; Cancer Biology; Cancer Center; Clinical; Clinical Data; Collaborations; Collection; Communities; Consent; Cryopreservation; DNA amplification; Data; Databases; Dedications; Detection; Diagnosis; Disease; Enrollment; Ensure; Epidemiology; Evolution; Freezing; Funding; Future; Genomic DNA; Genomics; Goals; Grant; Harvest; Immune system; Immunologics; Informatics; Institution; Institutional Review Boards; Investigation; Lesion; Liquid substance; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Metadata; Molecular; Newly Diagnosed; Normal tissue morphology; Operative Surgical Procedures; Organoids; Outcome; Pancreas; Pancreatic Ductal Adenocarcinoma; Pathogenesis; Pathologic; Pathologist; Pathology; Patient-Focused Outcomes; Patients; Peripheral Blood Mononuclear Cell; Plasma; Population; Prevention; Process; Proteins; Protocols documentation; Quality Control; RNA; Radiation; Research; Research Personnel; Resected; Resource Informatics; Resources; Sampling; Science; Serum; Solid; Solid Neoplasm; Source; Specimen; Testing; Time; Tissue Banks; Tissue Expansion; Tissue Procurements; Tissues; Transcript; Translational Research; Tumor Bank; Tumor Biology; Tumor Cell Line; Tumor Tissue; Universities; Urine; Washington; Xenograft procedure; anticancer research; biobank; bioinformatics infrastructure; biomedical informatics; cancer cell; cell free DNA; chemotherapy; clinical database; cohort; follow-up; improved; in vivo Model; individual patient; medical schools; neoplasm resource; neoplastic cell; novel strategies; pancreatic cancer patients; patient derived xenograft model; peripheral blood; premalignant; preservation; programs; prospective; research study; sample collection; success; therapy resistant; tissue culture; tissue processing; tissue registry; treatment response; tumor; tumor microenvironment; whole genome

PROJECT SUMMARY The controlled collection and processing of clinical specimens from patients with pancreatic ductal adenocarcinoma (PDAC) is a critical activity for an efficient and comprehensive program in translational research in the context of a SPORE grant. Accordingly, the Biospecimen Core (Core B) has one overarching aim: We will collect, store, process, and distribute biospecimens from patients with a diagnosis of PDAC seen at this institution to facilitate biospecimen-based translational research: We will collect malignant cell populations from tumors (from pre-surgical and surgical biopsies). We will also collect normal pancreas, pre-malignant pancreatic lesions, and peripheral blood from PDAC patients. Serum and plasma will be collected for correlative and future studies. Specimens will be collected throughout each patient's disease course (initial presentation, pre-treatment, post- treatment/follow-up), and, where appropriate, archival specimens from previous biopsies/etc. will be retrieved. Particular attention to specimen procurement (e.g. rapid processing of tissue to preserve transcript profiles) and quality control will be practiced. Specimens will be processed to cellular RNA, genomic DNA, whole genome amplified DNA, and protein extracts as required for each study. We will also collect longitudinal patient cell-free DNA samples for downstream analysis. Cellular populations will also be viably frozen or immediately processed for patient-derived xenograft and/or organoid creation. Importantly, we will ensure that all specimens used for research are extensively and accurately annotated with clinical (pre-treatment, treatment, and follow-up) data and metadata utilizing the bioinformatics infrastructure at our institution. Expert pathologic review from a dedicated GI pathologist will ensure high-quality annotation. The aims of this Core will be accomplished by continuing the scope of our well-established Cancer Center Tumor Bank and prior PDAC SPORE Core B successes and an on-going, funded effort to collect solid gastrointestinal malignancies at our institution. Specifically, Core B will expand the number of PDAC patients from whom biospecimens will be collected, and serve as a conduit (through data and specimen sharing) to allow for a broader variety of translational research studies in PDAC malignancies, using new and previously banked biospecimens.

项目总结