The KAPP-Sen Tissue Mapping Center Collaborative

KAPP-森组织绘图中心合作

• 批准号: 10376627
• 负责人: VESNA D GAROVIC
• 金额: $ 270万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10376627
• 关键词: Address; Adipose tissue; Age; Aging; Atlases; Bioinformatics; Biological; Biological Aging; Biological Markers; Biology; Biometry; Biopsy; Boston; Brain Death; Cell Aging; Cesarean section; Chronic Disease; Clinic; Clinical; Clinical Trials; Collaborations; Complex; Cytometry; Data; Data Analyses; Dermatology; Diabetes Mellitus; Discipline of obstetrics; Donor person; Elderly; Elements; Endocrinology; Evaluation; Fatty acid glycerol esters; Future; Generations; Genomic medicine; Genomics; Geriatrics; Geroscience; Goals; Gynecology; Health; Heart; Heterogeneity; Hospitals; Human; Image; Individual; Institution; Kidney; Kidney Transplantation; Knowledge; Laboratories; Nephrology; Outpatients; Pancreas; Pathology; Phase; Phenotype; Placenta; Positioning Attribute; Pregnancy; Program Evaluation; RLK5-associated protein phosphatase; Research; Research Activity; Research Design; Research Personnel; Resources; Risk Factors; Sample Size; Sampling; Site; Skin; Skin Tissue; Spatial Distribution; Specific qualifier value; Syndrome; Thick; Tissues; Translations; United States National Institutes of Health; Woman; design; flexibility; human subject; human tissue; innovation; insight; interest; modifiable risk; multidisciplinary; racial and ethnic; sample collection; scale up; senescence; sex; single cell technology; skills; telomere; transcriptomics; translational study

PROJECT SUMMARY OVERALL This proposal seeks to establish the KAPP-Sen Tissue Mapping Center Collaborative (TMC) as part of the Cellular Senescence Network: Tissue Mapping Centers effort {RFA-RM-21-008). Our application represents a multidisciplinary collaborative effort involving six leading institutions and aging research programs working together to characterize the distribution and biological heterogeneity of senescent cells in different healthy human tissues in full alignment with the objectives of the Cellular Senescence Network {SenNet). KAPP-Sen brings together skills, resources and perspectives needed to address Sen-Net goals in the framework of the healthy human Kidney. Adipose tissues, Pancreas and Placenta. Given our ability to obtain full thickness skin tissues from individuals providing KAPP samples, we may explore the possibility of future collaborative studies with TMCs selected for their major focus on skin. Our six collaborating institutions located in Farmington. CT (UConn Health, Jackson Laboratory for Genomic Medicine), Boston {Brigham & Women's Hospital, Joslin Diabetes Center), Rochester. MN {Mayo Clinic) and San Antonio. TX {UTHCSA) have all been carefully selected for their essential and unique individual contributions to the field and this effort. Our objectives will be achieved through the following aims: Aim 1 : Coordinate research activities across KAPP-Sen TMC Collaborative sites in support of Sen-Net goals towards mapping cellular senescence and its associated secretory phenotype in the healthy human kidney, adipose tissues, pancreas and placenta. Aim 2: Obtain tissues from healthy kidney transplant donors {kidneys, fat, skin), C-section pregnancies {placenta, cord, fat, skin), outpatient healthy donor biopsies {fat, skin), beating heart brain dead donors {full pancreas) and IIPD/Prodo {dispersed pancreas). Aim 3: Generate highest quality data pertaining to cellular senescence including scRNA-Seq, snRNA-Seq, spatial transcriptomics, immunohistochemistry and Telomere-associated DDR foci {TAFs) in all tissues Aim 4: Perform high level integrative data analysis required for the creation of aUases of human cellular senescence in collaboration with other TMCs, CODDC and NIH staff.

总体项目摘要 该建议旨在建立Kapp-Sen组织映射中心协作（TMC）作为细胞衰老网络的一部分：组织映射中心的工作{RFA-RM-21-008）。我们的应用代表了一项多学科的协作努力，涉及六个领先的机构和老化的研究计划，以共同表征不同健康人体组织中衰老细胞的分布和生物异质性，这与细胞衰老网络的目标完全一致（SENNET）。 Kapp-sen汇集了在健康人肾脏框架内解决SEN-NET目标所需的技能，资源和观点。脂肪组织，胰腺和胎盘。鉴于我们能够从提供KAPP样品的个体中获得全厚的皮肤组织，因此我们可能会探索与TMC选择将来的合作研究的可能性，以便将其主要关注皮肤。我们位于法明顿的六个合作机构。 CT（UConn Health，杰克逊基因组医学实验室），波士顿（乔斯林糖尿病中心的杨百翰及妇女医院），罗切斯特。 Mn（Mayo Clinic）和圣安东尼奥（San Antonio）。 TX {UTHCSA）都被仔细选择了他们对该领域的基本和独特的个人贡献和这项工作。 我们的目标将通过以下目的实现： AIM 1：协调KAPP-SEN TMC协作站点的研究活动，以支持sen-net目标，以绘制细胞衰老及其相关的分泌表型，及其在健康的人肾脏，脂肪组织，胰腺和胎盘中。 AIM 2：从健康的肾脏移植供体（肾脏，脂肪，皮肤），剖腹产（胎盘，脐带，脂肪，皮肤），门诊健康的供体活检（脂肪，皮肤），殴打心脏脑死亡供体（Full Pancreas）和IIPD/Prodo {分配的pancreas）中获取组织。 AIM 3：生成与细胞衰老有关的最高质量数据 AIM 4：与其他TMC，CODDC和NIH员工合作创建人类细胞衰老的AUASE所需的高级综合数据分析。