Molecular mechanisms controlling stress responses and cell adhesion in bacteria

控制细菌应激反应和细胞粘附的分子机制

• 批准号: 10380281
• 负责人: Sean Crosson
• 金额: $ 3.22万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10380281
• 关键词: Address; Adhesions; Anabolism; Bacteria; Bacterial Infections; Bacterial Physiology; Biochemical; Biology; Biophysics; Cell Adhesion; Cells; Chemicals; Clinical; Communities; Complex; Cytoplasm; Data; Detection; Environment; Gene Expression Regulation; Genetic; Growth; Infection; Investigation; Microbial Biofilms; Molecular; Molecular Structure; Physiology; Polysaccharides; Process; Route; Sensory; Signal Transduction; Stress; Structure; Surface; System; Virulence; Work; biological adaptation to stress; cell envelope; genetic regulatory protein; improved; novel therapeutics; pathogenic bacteria; response

Project Summary/Abstract My group seeks to understand molecular mechanisms that underlie the ability of bacterial cells to survive in complex, dynamic environments, including mammalian hosts. In the context of this project, we will specifically focus on defining mechanisms by which bacteria (i) regulate their physiology to survive environmental stress, and (ii) regulate and modify their envelope to control adhesion to surfaces and to other cells. We will utilize an interdisciplinary set of genetic, biochemical, biophysical, and computational approaches to address these questions on multiple scales, from the cellular/systems level to the level of molecular structure. The data that emerge from our studies will enhance understanding of processes that allow bacteria to grow and survive in complex environments, and will inform new concepts in gene regulation and cell envelope biology. More specifically, this project will provide the scientific community with an integrative understanding of sensory transduction mechanisms, from signal detection to cellular response. In addition, our investigations of bacterial cell adhesion and envelope polysaccharide biosynthesis will lead to improved understanding of the molecular mechanisms by which bacteria build the highly complex structure known as the envelope, which separates the tightly controlled activities in the cytoplasm from the outside world. Importantly, both environmental regulatory proteins and components of the bacterial cell envelope are well-defined virulence determinants in many bacterial pathogens. Thus our work has the potential to inform new therapeutic routes to control certain bacterial infections.

项目总结/摘要 我的团队寻求了解细菌细胞能力的分子机制 在复杂的动态环境中生存，包括哺乳动物宿主。结合这一 项目，我们将特别侧重于定义细菌（i）调节其 生理学以在环境压力下生存，以及（ii）调节和修改其包膜以控制 粘附于表面和其他细胞。我们将利用跨学科的遗传学， 生物化学、生物物理和计算方法来解决多个方面的这些问题 尺度，从细胞/系统水平到分子结构水平。出现的数据 将增强对细菌生长过程的理解， 在复杂环境中生存，并将为基因调控和细胞 包膜生物学更具体地说，该项目将为科学界提供一个 从信号检测到细胞的感觉传导机制的综合理解 反应此外，我们对细菌细胞粘附和包膜多糖的研究 生物合成将导致更好地理解分子机制， 细菌构建了高度复杂的结构，称为包膜，它将细菌与细菌紧密地分开。 控制细胞质中来自外界的活动重要的是，环境 细菌细胞包膜的调节蛋白和组分具有明确的毒力 许多细菌病原体中的决定因素。因此，我们的工作有可能为新的 控制某些细菌感染的治疗途径。