Olfactory Bulb Local Circuits
Olfactory Bulb Local Circuits
批准号:
10374882
负责人:
Charles A Greer
金额:
$35.59万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
已结题
起止时间:
2019-04-01 至 2024-03-31
关键词:
3-DimensionalActivities of Daily LivingAddressAdultAffectAgeAmacrine CellsApicalAttentionAxodendritic SynapseAxonBackBasal CellBiophysicsBrainCell LineageCellsCharacteristicsCodeComplementDataDendritesDendritic SpinesDendrodendritic SynapseDevelopmentElectron MicroscopyElectroporationEmbryoFOS geneFoundationsGenetic RecombinationGoalsImmunoelectron MicroscopyIndividualInterneuronsLabelLigand BindingLightLiteratureMapsMediatingMolecularMorphologyMusNervous system structureNeuronsOdorant ReceptorsOdorsOlfactory EpitheliumOlfactory PathwaysPerinatalPhenotypePlasmidsPopulationPopulation ProjectionProceduresProcessPropertyProteinsRecording of previous eventsRegulationReportingResearchRetinal Ganglion CellsRoleSeriesShapesSmell PerceptionSpatial DistributionSpecificitySynapsesSynaptic VesiclesTamoxifenTechniquesTestingThymidineVisual PathwaysWorkanalogbaseexperimental studyfluorophoregranule cellimmunohistochemical markersin uteroinnovationinsightlight microscopymitral cellneural circuitneurogenesisneuronal cell bodynovel strategiesolfactory bulbolfactory bulb glomeruliolfactory sensory neuronspostnatalreceptorreconstructionsensory systemsoundstem cellssynaptic functionsynaptogenesissystematic review
中文摘要
项目摘要-摘要
当气味配体结合在嗅觉上皮细胞上时,气味的感知开始于嗅觉上皮细胞。
感觉神经元,其中每一个仅表达约1200个候选受体中的一个。轴突来自
表达相同气味受体的神经元仅会聚到2/3的肾小球/嗅球中,
它们与投射神经元形成突触,每个投射神经元支配单个肾小球。深至
在肾小球外丛状层(EPL)内,气味编码由局部突触回路调节。
破译与EPL的连接是理解中枢神经系统机制的第一步。
气味处理已经提出了几种EPL中的局部加工假设,
突触组织通常被认为是典型的。然而,我们缺乏对
中间神经元、颗粒细胞(GC)、结构、分子和地形的多样性
组织影响EPL局部电路,这是解释功能研究的障碍。这里我们
就可能影响组织的因素提出一系列可检验的假设和实验
和它们的局部突触回路。这些研究建立在我们先前的发展和
超微结构工作以及对当前文献的系统综述,并认识到,
EPL组织和连通性的基本特征已经被假定,但不是经验性的
测试.首先,我们提出的假设,即克隆的历史,时间和顺序的GC
神经发生是它们在嗅球中的组织/分布的决定因素。使用多个
追踪神经发生、细胞谱系/命运的策略,我们将评估GC的多刺树突状乔木
从胚胎开始,以固定的间隔持续到200天的年龄。在
此外,而不是在以前的研究中,我们建议仔细评估GC基底的组织,
树突是传入离心调制的主要接受者(Rothermei和
Wachowiak,2014; Kapoor等人,2016; de Almeida等人,2015)。第二,我们建议测试
EPL中树-树突触的突触学和分子特征不同的假说
as a function函数of age年龄.目前,很少有人知道的结构特点,调节树突状细胞
突触或二尖瓣与GC的分子特性以及GC与二尖瓣细胞的相互作用
突触可能不同。这些分析将解决这一根本问题,并提供一个健全的
气味处理功能分析的解释基础。此外,我们将解决
突触并置沿着2 o树突的空间分布以及突触并置的特征
突触特化随年龄变化。因为我们要解决的问题是
重要的是在整个神经系统,我们预计,结果将有广泛的影响,
了解整个大脑神经元的靶向、层状特异性和突触连接。
英文摘要
Project Summary – Abstract
The perception of odors begins in the olfactory epithelium when odorant ligands bind on olfactory
sensory neurons, each of which expresses only 1 of ~1200 candidate receptors. Axons coming from
neurons expressing the same odorant receptor converge into only 2/3 glomeruli/olfactory bulb where
they synapse onto projection neurons, each of which innervates a single glomerulus. Deep to the
glomeruli, within the external plexiform layer (EPL), odor coding is tuned by local synaptic circuits.
Deciphering connectivity with the EPL is the first step toward understanding the mechanisms of central
odor processing. Several hypotheses of local processing within the EPL have been proposed and its
synaptic organization is often presented as canonical. However, we lack a fundamental understanding of
the how the diversity in interneuron, granule cell (GC), structural, molecular, and topographical
organization affects EPL local circuits, which is an impediment to interpreting functional studies. Here we
propose a series of testable hypotheses and experiments on factors that may influence the organization
of GCs and their local synaptic circuits. These studies build on our prior developmental and
ultrastructural work as well as a systematic review of the current literature and the recognition that
fundamental features of EPL organization and connectivity have been presumed, but not empirically
tested. First, we propose to address the hypotheses that the clonal history, timing and order of GC
neurogenesis are determinants of their organization/distribution in the olfactory bulb. Using multiple
strategies to track neurogenesis, cell lineage/fate we will assess the spiny dendritic arbors of GCs
beginning in the embryo and continuing at regular intervals to those generated up 200 days of age. In
addition, and not among prior studies, we propose to carefully assess the organization of the GC basal
dendrites which are the primary recipients of incoming centrifugal modulation (Rothermei and
Wachowiak, 2014; Kapoor et al., 2016; de Almeida et al., 2015). Second, we propose to test the
hypothesis that the synaptology and molecular features of the dendrodendritic synapses in the EPL vary
as a function of age. Presently, little is known of the structural features that regulate dendrodendritic
synapses or how the molecular properties of the mitral to GC and the reciprocal GC to mitral cell
synapses may differ. The analyses will address that fundamental problem and provide a sound
foundation for the interpretation of functional analyses of odor processing. In addition, we will address
the spatial distribution of synaptic appositions along 2o dendrites and the degree to which the features of
the synaptic specialization change with age. Because the questions we propose to address are
important throughout the nervous system, we anticipate that the results will have broad implications for
understanding targeting, laminar specificity, and synaptic connectivity of neurons throughout the brain.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Dynamic interactions among olfactory sensory neuron axons
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批准号:10224737
-
项目类别:
-
资助金额:$41.48万
-
财政年份:2019
-
负责人:Charles A Greer
-
依托单位:
Olfactory Bulb Local Circuits
-
批准号:9888351
-
项目类别:
-
资助金额:$35.59万
-
财政年份:2019
-
负责人:Charles A Greer
-
依托单位:
Dynamic interactions among olfactory sensory neuron axons
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批准号:10685631
-
项目类别:
-
资助金额:$41.48万
-
财政年份:2019
-
负责人:Charles A Greer
-
依托单位:
Olfactory Bulb Local Circuits
-
批准号:10604323
-
项目类别:
-
资助金额:$35.59万
-
财政年份:2019
-
负责人:Charles A Greer
-
依托单位:
Piriform cortex: sequential developmental events
-
批准号:10589155
-
项目类别:
-
资助金额:$41.88万
-
财政年份:2014
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负责人:Charles A Greer
-
依托单位:
Piriform cortex: sequential developmental events
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批准号:10442245
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项目类别:
-
资助金额:$41.88万
-
财政年份:2014
-
负责人:Charles A Greer
-
依托单位:
Piriform cortex: sequential developmental events
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批准号:9171950
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项目类别:
-
资助金额:$35.38万
-
财政年份:2014
-
负责人:Charles A Greer
-
依托单位:
Piriform cortex: sequential developmental events
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批准号:8957912
-
项目类别:
-
资助金额:$35.38万
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财政年份:2014
-
负责人:Charles A Greer
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依托单位:
The Olfactory Nerve
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批准号:9118166
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项目类别:
-
资助金额:$35.14万
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财政年份:2012
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负责人:Charles A Greer
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依托单位:
The Olfactory Nerve
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批准号:8904648
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项目类别:
-
资助金额:$34.79万
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财政年份:2012
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负责人:Charles A Greer
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依托单位:
The Olfactory Nerve
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批准号:8433643
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项目类别:
-
资助金额:$36.71万
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财政年份:2012
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负责人:Charles A Greer
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依托单位:
The Olfactory Nerve
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批准号:8706121
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项目类别:
-
资助金额:$35.14万
-
财政年份:2012
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负责人:Charles A Greer
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依托单位:
The Olfactory Nerve
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批准号:8545154
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项目类别:
-
资助金额:$33.39万
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财政年份:2012
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负责人:Charles A Greer
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依托单位:
Mechanisms of Aging in the Olfactory System
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批准号:7477360
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项目类别:
-
资助金额:$125.61万
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财政年份:2006
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负责人:Charles A Greer
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依托单位:
Neuron Glial Interactions
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批准号:7627341
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项目类别:
-
资助金额:$29.12万
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财政年份:2006
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负责人:Charles A Greer
-
依托单位:
Mechanisms of Aging in the Olfactory System
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批准号:7084154
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项目类别:
-
资助金额:$130.99万
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财政年份:2006
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负责人:Charles A Greer
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依托单位:
Neuron Glial Interactions
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批准号:7442302
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项目类别:
-
资助金额:$28.27万
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财政年份:2006
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负责人:Charles A Greer
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依托单位:
Neuron Glial Interactions
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批准号:7145727
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项目类别:
-
资助金额:$33.77万
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财政年份:2006
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负责人:Charles A Greer
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依托单位:
Mechanisms of Aging in the Olfactory System
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批准号:7286255
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项目类别:
-
资助金额:$124.03万
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财政年份:2006
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负责人:Charles A Greer
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依托单位:
Neuron Glial Interactions
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批准号:7248591
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项目类别:
-
资助金额:$31.65万
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财政年份:2006
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负责人:Charles A Greer
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依托单位:
海外基金