Dynamic interactions among olfactory sensory neuron axons

嗅觉感觉神经元轴突之间的动态相互作用

基本信息

  • 批准号:
    10685631
  • 负责人:
  • 金额:
    $ 41.48万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-09-01 至 2024-08-31
  • 项目状态:
    已结题

项目摘要

Project Summary – Abstract Odor perception begins in the olfactory epithelium (OE) when ligands bind to molecular receptors expressed on the cilia of the olfactory sensory neurons (OSNs). Buck and Axel (1991) were the first to describe the large family of genes coding for the odorant receptors (ORs), now known to number ~1,200 in mice. An OSN expresses only 1 OR. OSNs expressing the same OR do not cluster but rather are broadly distributed across the OE. Thus, the OE is a complex mosaic of neurons each of which expresses only 1 of 1,200 possible ORs. As OSN axons exit the OE they initially fasciculate with nearest neighbors, not necessarily with axons from OSNs expressing the same OR. However, as they progress over the surface of the olfactory bulb to a point of glomerular convergence, the axons undergo a profound topographical reorganization such that all of the axons coming from neurons expressing the same OR converge into only 2/3 glomeruli/olfactory bulb. This process of reorganization of axons and convergence into specific glomeruli is broadly conserved among vertebrates and poses a significant wiring problem, perhaps the most complex wiring problem found among sensory systems. Despite concerted efforts to identify the molecular substrates of OSN axon growth, coalescence and targeting, we remain woefully ignorant of the most fundamental aspects of axon:axon interactions: How does the reorganization of OSN axons relate to the organization of their axoskeleton and organelles? What are the axoskeleton dynamics as axons initially fasciculate and extend toward the OB and when they defasciculate in the olfactory nerve layer, forming new OR homotypic fascicles targeted to specific glomerului? What drives fasciculation/defasciculation of OSN axons; are mechanical forces involved? When do we recognize homotypic fasciculation? What is the timeline for the maturation of OSNs and how does it relate to the extension of the axon to OB targets and functional activity? Importantly, these fundamental questions apply equally to all vertebrates, in which olfactory system development obeys the same basic rules. Thus here, in addition to studies of axon:axon interactions and ultrastructure in mice, we introduce a new model, live imaging in zebrafish, to assess the dynamic nature of OSN axon:axon interactions during development. To begin addressing these significant gaps in our knowledge we propose 3 specific aims: Aim 1 - Test hypotheses regarding the cytoskeletal organization of OSN axons and their fasciculation in the inner and outer sublaminae of the olfactory nerve layer of the olfactory bulb. Aim 2 – Test the hypotheses that the axoskeleton dynamics, as well as mechanical forces, control the fasciculation/defasciculation and navigation of OSN axons in the live zebrafish. Aim 3 – Test the hypothesis that the spatio-temporal dynamics of OSN axon extension and the expression of cytoskeletal and adhesion molecules differ in perinatal versus adult mice.
项目摘要-摘要

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Actomyosin contractility in olfactory placode neurons opens the skin epithelium to form the zebrafish nostril.
嗅板神经元中的肌动球蛋白收缩性打开皮肤上皮形成斑马鱼鼻孔。
  • DOI:
    10.1016/j.devcel.2023.02.001
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    11.8
  • 作者:
    Baraban,Marion;GordilloPi,Clara;Bonnet,Isabelle;Gilles,Jean-François;Lejeune,Camille;Cabrera,Mélody;Tep,Florian;Breau,MarieAnne
  • 通讯作者:
    Breau,MarieAnne
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Charles A Greer其他文献

Charles A Greer的其他文献

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{{ truncateString('Charles A Greer', 18)}}的其他基金

Dynamic interactions among olfactory sensory neuron axons
嗅觉感觉神经元轴突之间的动态相互作用
  • 批准号:
    10224737
  • 财政年份:
    2019
  • 资助金额:
    $ 41.48万
  • 项目类别:
Olfactory Bulb Local Circuits
嗅球局部电路
  • 批准号:
    9888351
  • 财政年份:
    2019
  • 资助金额:
    $ 41.48万
  • 项目类别:
Olfactory Bulb Local Circuits
嗅球局部电路
  • 批准号:
    10604323
  • 财政年份:
    2019
  • 资助金额:
    $ 41.48万
  • 项目类别:
Olfactory Bulb Local Circuits
嗅球局部电路
  • 批准号:
    10374882
  • 财政年份:
    2019
  • 资助金额:
    $ 41.48万
  • 项目类别:
Piriform cortex: sequential developmental events
梨状皮层:顺序发育事件
  • 批准号:
    10589155
  • 财政年份:
    2014
  • 资助金额:
    $ 41.48万
  • 项目类别:
Piriform cortex: sequential developmental events
梨状皮层:顺序发育事件
  • 批准号:
    10442245
  • 财政年份:
    2014
  • 资助金额:
    $ 41.48万
  • 项目类别:
Piriform cortex: sequential developmental events
梨状皮层:顺序发育事件
  • 批准号:
    9171950
  • 财政年份:
    2014
  • 资助金额:
    $ 41.48万
  • 项目类别:
Piriform cortex: sequential developmental events
梨状皮层:顺序发育事件
  • 批准号:
    8957912
  • 财政年份:
    2014
  • 资助金额:
    $ 41.48万
  • 项目类别:
The Olfactory Nerve
嗅觉神经
  • 批准号:
    9118166
  • 财政年份:
    2012
  • 资助金额:
    $ 41.48万
  • 项目类别:
The Olfactory Nerve
嗅觉神经
  • 批准号:
    8904648
  • 财政年份:
    2012
  • 资助金额:
    $ 41.48万
  • 项目类别:

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