Mutant Mouse/Viral Vector Core

突变小鼠/病毒载体核心

• 批准号: 10374824
• 负责人: ARON H LICHTMAN
• 金额: $ 30.58万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10374824
• 关键词: Animals; Automobile Driving; Biological; Brain region; Breeding; CRISPR/Cas technology; Collaborations; Communities; Complex; Consultations; DNA cassette; Derivation procedure; Development; Dominant-Negative Mutation; Drug Modelings; Drug abuse; Embryo Transfer; Ensure; Enterobacteria phage P1 Cre recombinase; Environmental Risk Factor; Event; Experimental Designs; Future; Gene Deletion; Gene Delivery; Gene Targeting; Gene Transfer Techniques; Gene-Modified; Generations; Genes; Genetic; Genetically Engineered Mouse; Genomics; Genotype; Goals; Immunohistochemistry; Injections; Institution; Knock-in; LoxP-flanked allele; Maintenance; Measures; Modeling; Molecular; Mus; Mutant Strains Mice; Outcome; Outcome Study; Pharmaceutical Preparations; Point Mutation; Production; Quality Control; Research; Research Personnel; Research Project Grants; Robotics; Scientist; Services; Signal Transduction; Source; Talents; Techniques; Technology; Training; Training and Infrastructure; Transgenic Model; Transgenic Organisms; Universities; Validation; Viral Vector; Virginia; Weaning; addiction; adeno-associated viral vector; behavioral pharmacology; brain cell; cell type; cryogenics; delivery vehicle; design; drug of abuse; embryo cryopreservation; interest; knock-down; member; model development; mouse model; mutant; neural circuit; novel; optogenetics; overexpression; pathogen; preservation; promoter; ranpirnase; repository; response; small hairpin RNA; sperm cryopreservation; targeted delivery; therapeutic target; tool; transcriptome sequencing; vector

Project Summary – Mutant Mouse/Viral Vector Core The objective of this Core is to provide expert advice from the talented team of co-investigators, necessary training, and the infrastructure to utilize molecular biological approaches to create genetically engineered mouse models that will increase the depth and breadth of existing drug abuse research and stimulate new research at Virginia Commonwealth University and throughout the scientific community. Additionally, it will provide a mechanism for scientists both within and outside of the drug abuse field to interact to develop new research projects. Specifically, this Core will provide investigators the necessary training, tools, and expertise to: 1) create novel genetically modified mice, not currently available through other sources; 2) maintain mice in a repository in which available mouse lines will be bred, genotyped, and transferred to the investigator upon weaning; 3) cryogenically preserve mouse lines for use in future studies; 4) develop viral- vectors to over-express, knock-down or deliver dominant negative forms of study genes chosen by collaborating investigators; and· 5) deliver viral vectors stereotaxically into specific brain regions of interest and verify the extent of the manipulation. Overall, this Core will provide new services to multiple investigators, with the goals of providing state-of-the-art molecular biological tools to investigate drugs of abuse, facilitate collaborations, and provide value to drug abuse researchers.

项目总结-突变小鼠/病毒载体核心 该核心小组的目标是，在必要时， 培训和基础设施，利用分子生物学方法来创造基因工程 小鼠模型将增加现有药物滥用研究的深度和广度并刺激新的药物滥用研究 弗吉尼亚联邦大学和整个科学界的研究。此外，它将 为药物滥用领域内外的科学家提供一个相互作用的机制， 研究项目。具体而言，该核心将为研究者提供必要的培训、工具， 专业知识：1）创造新的转基因小鼠，目前无法通过其他来源获得; 2） 将小鼠保存在储存库中，在储存库中对可用的小鼠品系进行繁殖、基因分型，并转移到 研究者在断奶后; 3）低温保存小鼠品系用于未来研究; 4）培养病毒- 载体过表达，敲低或传递显性阴性形式的研究基因， · 5）立体定向地将病毒载体递送到感兴趣的特定脑区域 并核实操纵的程度。总体而言，该核心将为多名研究者提供新的服务， 目的是提供最先进的分子生物学工具来调查药物滥用， 合作，并为药物滥用研究人员提供价值。