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Mutant Mouse/Viral Vector Core

突变小鼠/病毒载体核心

基本信息

批准号：
10604268
负责人：
ARON H LICHTMAN
金额：
$ 30.58万
依托单位：
VIRGINIA COMMONWEALTH UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-12-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10604268
关键词：
Animal Experiments Animals Automobile Driving Biological Brain region Breeding CRISPR/Cas technology Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Communities Complex Consultations DNA cassette Derivation procedure Development Dominant-Negative Mutation Drug Modelings Drug abuse Embryo Transfer Ensure Enterobacteria phage P1 Cre recombinase Environmental Risk Factor Event Experimental Designs Future Gene Deletion Gene Delivery Gene Modified Gene Targeting Gene Transfer Techniques Generations Genes Genetic Genetically Engineered Mouse Genomics Genotype Goals Immunohistochemistry Infrastructure Injections Institution Knock-in LoxP-flanked allele Maintenance Measures Modeling Molecular Mus Mutant Strains Mice Outcome Outcome Study Outsourcing Pharmaceutical Preparations Point Mutation Production Quality Control Research Research Personnel Research Project Grants Robotics Scientist Services Signal Transduction Source Talents Techniques Technology Training Transgenic Model Transgenic Organisms Universities Validation Viral Vector Virginia Weaning addiction adeno-associated viral vector behavioral pharmacology brain cell cell type cryogenics delivery vehicle design drug of abuse embryo cryopreservation genetic approach interest knock-down member model development mouse model mutant neural circuit novel optogenetics overexpression pathogen preservation promoter repository response small hairpin RNA sperm cryopreservation targeted delivery therapeutic target tool transcriptome sequencing vector

项目摘要

Project Summary – Mutant Mouse/Viral Vector Core The objective of this Core is to provide expert advice from the talented team of co-investigators, necessary training, and the infrastructure to utilize molecular biological approaches to create genetically engineered mouse models that will increase the depth and breadth of existing drug abuse research and stimulate new research at Virginia Commonwealth University and throughout the scientific community. Additionally, it will provide a mechanism for scientists both within and outside of the drug abuse field to interact to develop new research projects. Specifically, this Core will provide investigators the necessary training, tools, and expertise to: 1) create novel genetically modified mice, not currently available through other sources; 2) maintain mice in a repository in which available mouse lines will be bred, genotyped, and transferred to the investigator upon weaning; 3) cryogenically preserve mouse lines for use in future studies; 4) develop viral- vectors to over-express, knock-down or deliver dominant negative forms of study genes chosen by collaborating investigators; and· 5) deliver viral vectors stereotaxically into specific brain regions of interest and verify the extent of the manipulation. Overall, this Core will provide new services to multiple investigators, with the goals of providing state-of-the-art molecular biological tools to investigate drugs of abuse, facilitate collaborations, and provide value to drug abuse researchers.

项目摘要-突变小鼠/病毒载体核心这一核心的目标是在必要时从有才华的协查团队中提供专家意见培训，以及利用分子生物学方法创造基因工程的基础设施将增加现有药物滥用研究的深度和广度并刺激新的弗吉尼亚联邦大学和整个科学界的研究。此外，它还将为药物滥用领域内外的科学家提供一种互动机制，以开发新的研究项目。具体地说，该核心将为调查人员提供必要的培训、工具和专业知识：1)创造目前通过其他来源无法获得的新型转基因小鼠；2) 在储存库中维护小鼠，在该储存库中将培育可用小鼠品系，对其进行基因分型，并将其转移到断奶后的研究人员；3)冷冻保存小鼠品系，以供未来研究使用；4)开发病毒- 载体以过度表达、敲除或传递显性阴性形式的研究基因，由合作研究人员；以及·5)将病毒载体立体定向地传递到特定的大脑感兴趣区域并核实操纵的程度。总体而言，这个核心将为多个调查人员提供新的服务，以提供最先进的分子生物学工具来调查滥用药物为目标，促进合作，并为药物滥用研究人员提供价值。