Core 2: Genomics

摘要 基因组学核心(核心2)将为基因组图谱和综合生物信息学提供支助。 对所有四个项目的分析支持，有助于实现方案的总体目标，以了解 BRCA网络在DNA损伤修复和复制保真度中的调节机制 保持基因组的完整性以抑制肿瘤的发生。基因组学核心将由Subhajyoti博士领导 De，PhD，国际知名的基因组科学家，有10年的工作经验 癌症基因组学和DNA修复。他的实验室是一家设备齐全的最先进的基因组设备，配备了专家 专门从事基因组学和生物信息学分析的人员，包括全基因组测序、RNA- SEQ、CHIP-SEQ、ATAC-SEQ和染色质构象测序方法。德博士广泛地 在这个P01项目上与主要调查人员合作，从共同的恩赐精神和 出版物。张陈博士，这一核心的共同研究员，也是基因组学的联合领导者 罗格斯癌症研究所与沈博士共同开展的不稳定性和癌症基因组计划，并 与P01调查人员广泛合作。王亚群博士，博士，另一位合作调查员 与P01调查人员的合作。Core已经从小鼠肿瘤中产生了初步数据， 确定基因组变化，并进行初步数据分析。基因组学核心的具体目标 (核心2)是：1)使用基因组学方法确定复杂基因组重排的架构，(2) 诠释癌症基因组中复杂的染色体重排，以及(Iii)进行整合 BRCA网络的生物信息学分析。基因组学核心的两个创新方面是(I)使用 确定癌症基因组中复杂重排模式的新兴基因组技术，以及(Ii) 使用结构重排的序列和表观基因组学背景对它们进行注释和预测 潜在的病因。核心2将是所有计划演示文稿、报告和 试图了解BRCA网络在复制保真度和 DNA损伤修复。

ABSTRACT The Genomics Core (Core 2) will provide support with genomics profiling and integrative bioinformatics analysis support for all the four projects, contributing towards the Program's overall goal to understand the regulatory mechanisms of the BRCA network in DNA damage repair and replication fidelity, and the maintenance of genomic integrity to suppress tumorigenesis. The Genomics Core will be led by Dr. Subhajyoti De, PhD, who is an internationally known genomics scientist with >10 years experience working in the area of cancer genomics and DNA repair. His lab is a state-of-the-art fully equipped genomic facility staffed with expert personnel specializing in genomics and bioinformatics analysis including whole genome sequencing, RNA- Seq, ChIP-Seq, ATAC-Seq, and chromatin conformation sequencing approaches. Dr. De has extensively collaborated with the principal investigators on this P01 project, as evident from shared grantsmanship and publications. Dr. Chang Chan, PhD, co-investigator on this core is also the co-leader of the Genomics Instability and Cancer Genomics program at the Rutgers Cancer Institute jointly with Dr. Shen, and has extensive collaborations with the P01 investigators. Dr. Yaqun Wang, PhD, the other co-investigator has close collaborations with the P01 investigators. The Core has generated preliminary data from the mouse tumors, identified genomic alterations, and performed initial data analysis. The specific aims of the Genomics Core (Core 2) are: 1) identify architecture of complex genomic rearrangements using genomics approaches, (ii) annotate complex chromosomal rearrangements in cancer genomes, and (iii) perform integrative bioinformatics analysis of BRCA network. Two innovative aspects of the Genomics Core are (i) usage of emerging genomic technologies to determine patterns of complex rearrangements in cancer genomes, and (ii) use both sequence and epigenomic contexts of the structural rearrangements to annotate them and predict underlying etiologies. Core 2 will be central to the preparation of all Program presentations, reports, and manuscripts that seek to understand the regulatory mechanisms of the BRCA network in replication fidelity and DNA damage repair.