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Viral inhibition of cell death in host immune responses

病毒抑制宿主免疫反应中的细胞死亡

基本信息

批准号：
10397097
负责人：
Edward A Miao
金额：
$ 58.81万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-22 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10397097
关键词：
Apoptosis Apoptosis Inhibitor Binding Biological Process CASP8 gene CUL1 gene Caspase Inhibitor Cell Death Cell Death Inhibition Cells Co-Immunoprecipitations Complex Cowpox virus Cullin Proteins Defense Mechanisms Detection Disease Enzymes Exhibits F Box Domain Family Goals Herpesviridae Human Immune Immune Evasion Immune response Immune system Immunoprecipitation Infection Infiltration Inflammation Inflammatory Inflammatory Response Innate Immune Response Knowledge Light Lytic Mammalian Cell Maps Mediating Medical Modeling Molecular Mus NF-kappa B Orthopoxvirus Outcome Pathology Pathway interactions Pattern Phosphotransferases Poxviridae Protein Kinase Proteomics RIPK1 gene RIPK3 gene Recombinants Resistance Role Site Small Interfering RNA Testing Tissues Transfection Ubiquitination Vaccines Vaccinia virus Viral Viral Interference Viral Load result Viral Physiology Virus Virus Diseases Virus Inhibitors Virus Replication Western Blotting Work base cytokine design domain mapping immunopathology in vivo inhibitor insight multicatalytic endopeptidase complex mutant novel pathogenic virus response

项目摘要

Abstract/Summary of Work Cell death is a powerful immune defense mechanism to limit viral replication within the infected host. In response, viruses often encode inhibitors that counteract host cell death. For example, many herpesviruses and poxviruses encode inhibitors of caspases, the key enzymes that execute apoptosis. By subverting host cell death and the associated inflammatory response, viral cell death inhibitors can also dramatically alter the outcome of viral diseases. Necroptosis is a lytic form of cell death that is optimally induced in the presence of caspase 8 inhibition. As such, necroptosis has critical roles in controlling viruses that encode caspase inhibitors. Moreover, the release of “damage-associated molecular patterns (DAMPs)” from necroptotic cells can further stimulate anti- viral inflammation. These anti-viral effects suggest that necroptosis may also be a target of viral inhibition. To test this hypothesis, we conducted a siRNA screen and found a viral inhibitor from cowpox virus that we have termed “viral inducer of RIPK3 degradation (vIRD)”. In preliminary studies, we found that vIRD functions by targeting the essential necroptosis kinase RIPK3 for proteasomal degradation. Based on these observations, we propose three aims to further elucidate the function and mechanism of vIRD. In the first aim, we will evaluate the mechanism by which vIRD from cowpox virus and other related orthopoxviruses promotes RIPK3 degradation. Studies will include mapping the domain and residues within vIRD and RIPK3 that are responsible for their physical interaction and ubiquitination. In the second aim, we will interrogate the role of the host SKP1-Cullin-F box (SCF) complex in vIRD-mediated RIPK3 degradation. We will also evaluate the effect of vIRD on the global ubiquitination landscape of the infected cell. In Aim 3, we will evaluate the biological function of vIRD using mouse infection as model. We will infect mice with cowpox virus or vaccinia virus that differ in their expression of vIRD. The effect of vIRD on cell death, inflammation, viral replication and tissue pathology will be examined. Collectively, these studies will reveal medically relevant information on how viruses manipulate the immune system and alter the outcome of viral disease. We expect the knowledge gained from the proposed study will provide important insight in the design of better and more efficacious vaccines.

摘要/工作总结