Viral Vector Core

病毒载体核心

• 批准号: 9789006
• 负责人: JOEL R CHAMBERLAIN
• 金额: $ 23.72万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9789006
• 关键词: Animal Model; Animals; Antibodies; Basic Science; Biology; Biotechnology; Canis familiaris; Cardiac Myocytes; Cell Culture Techniques; Cell Line; Cells; Centrifugation; Collaborations; Complementary DNA; Consult; Consumption; Cytokeratin-8 Staining Method; Cytomegalovirus; Doctor of Philosophy; Dystrophin; Family suidae; Funding; Gene Transduction Agent; Gene Transfer; Genes; Hand; High Pressure Liquid Chromatography; Human Resources; Immune Sera; Laboratories; Learning; Lentivirus Vector; Methods; Muscle; Muscular Dystrophies; Myoblasts; Myopathy; Plasmid Cloning Vector; Plasmids; Preparation; Procedures; Production; Protein Isoforms; Protocols documentation; RNAi vector; Reagent; Recombinants; Regulator Genes; Research; Research Personnel; Resources; Retroviral Vector; Role; Roller Bottle; Serotyping; Services; Stem cells; Technology; Testing; Time; Training; Transfection; Translational Research; Utrophin; Vertebral column; Viral; Viral Vector; Visit; Work; adeno-associated viral vector; base; cost; gene therapy; improved; induced pluripotent stem cell; interest; member; mouse model; operation; outreach; research and development; research study; therapeutic development; vector; web site

VIRAL VECTOR CORE: Summary/Abstract This Viral Vector Core has been in operation for nearly 12 years, and its main role has been to support the Wellstone Center in Seattle as a Scientific Research Resource Core. The Core also offers support to non-Center members on a recharge basis. The Core provides high quality preparations of viral and plasmid vectors to facilitate research related to the muscular dystrophies. Services offered by the Core include providing consulting, reagents and training related to vector production. This includes production of adeno-associated and lentiviral vectors and muscular dystrophy related reagents (cell lines, vector backbones, protocols, antibodies etc) for members of this Center as well as laboratories in the US and across the World to support their efforts related to gene therapy for the muscular dystrophies. The Principle Investigator of the Vector Core is now Dr. Joel Chamberlain, who has worked closely with Core personnel on AAV and retroviral vectors for more than 10 years. The manager of the Core is Dr. James Allen. Dr. Allen, in conjunction with the Chamberlain, Russel, Odom and Hauschka labs has established protocols for the production of adeno-associated viral vectors, particularly AAV6 and 9, but also several other pseudotyped vectors including AAV1, 5, 7,8 and 8i, and VSVG-lentiviral vectors. For many years such vectors and associated reagents have been supplied by us to laboratories around the world as part of collaborations, and for the past 3.5 years (and a previous 5 year period) these reagents have been provided via the Wellstone Center Vector Core lab. We also have numerous dystrophin and utrophin cDNAs and antibodies that will be made available via this Core lab, as well as DM1, DUX4 and associated RNAi vectors. We will provide high quality, high titer preparations of adeno-associated viral (AAV) vectors based primarily on AAV serotypes 6 & 9, but also for AAV 1, 2, 8 and emerging new and modified serotypes. These recombinant AAV (rAAV) vectors will be provided to members of the Wellstone Center using funds from this Core, and to other muscular dystrophy researchers on a recharge basis to cover costs. We also have numerous muscle disease related cDNAs and antibodies that will be made available via this Core lab for all members of our Center. Training will allow investigators to apply methods for production and purification of viral vectors (AAV and lentiviral) in their own labs as desired, and will be advertised via the Center Web site. Consulting will be available to guide the use of the AAV and lentiviral vectors. Finally, we will provide reagents (such as vector backbones, plasmids, antibodies and protocols) that have been developed by members of this Wellstone Center to outside labs.

病毒矢量核心：摘要/摘要 该病毒矢量核心已经运行了将近12年，其主要作用是支持 西雅图的井石中心是科学研究资源核心。核心还为 在充电基础上进行非中心成员。核心提供了病毒和高质量的准备工作 质粒向量促进与肌肉营养不良有关的研究。核心提供的服务 包括提供与矢量生产有关的咨询，试剂和培训。这包括生产 腺相关和慢病毒载体以及肌肉营养不良相关的试剂（细胞系，载体） 该中心成员以及美国的实验室和实验室以及 全世界支持他们与肌肉营养不良的基因疗法有关的努力。这 矢量核心的主要调查员现在是乔尔·张伯伦（Joel Chamberlain）博士，他与 AAV和逆转录病毒载体上的核心人员已有10多年的历史。核心的经理是博士。 詹姆斯·艾伦。艾伦博士与张伯伦，罗素，奥多姆和豪施卡实验室结合 建立的用于生产腺相关病毒载体的方案，特别是AAV6和9，但 还有其他几种假型向量，包括AAV1，5、7,8和8i和VSVG慢性病毒载体。为了 多年以来，美国已经向实验室提供了此类矢量和相关试剂 世界作为合作的一部分，在过去的3。5年（和前5年）中，这些试剂 已经通过Wellstone Center Vector Core Lab提供。我们也有许多肌营养不良蛋白和 通过此核心实验室以及DM1，DUX4和 相关的RNAi向量。我们将提供与腺相关病毒的高质量高滴度制剂 （AAV）向量主要基于AAV血清型6和9，但也基于AAV 1、2、8和新兴 和修改的血清型。这些重组AAV（RAAV）向量将提供给 井石中心使用该核心的资金，以及其他肌肉营养不良研究人员 补给基础以支付费用。我们也有许多与肌肉疾病相关的cDNA和抗体 这将通过此核心实验室为我们中心的所有成员提供。培训将允许调查人员 将病毒载体（AAV和慢病毒）生产和纯化的方法应用于自己的实验室 期望，并将通过中心网站进行广告宣传。咨询将用于指导使用 AAV和慢病毒载体。最后，我们将提供试剂（例如矢量骨架，质粒， 该井中心成员已开发到外部的抗体和协议） 实验室。