Shared Resources Core

共享资源核心

• 批准号: 10732989
• 负责人: FENG CHEN
• 金额: $ 14万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10732989
• 关键词: ATAC-seq; Address; Anatomy; Atlases; Bioinformatics; Biological Assay; Biological Markers; Cells; Characteristics; Clinical; Complex; DNA; Data; Development; Drug resistance; Effectiveness; Ensure; Future; Genitourinary system; Genomics; Goals; Heterogeneity; Human; Image; Immunofluorescence Immunologic; Individual; Infrastructure; Maps; Mission; Modeling; Molecular; Molecular Profiling; Monitor; Oncogenes; Predictive Value; Procedures; Productivity; Proteins; Proteomics; RNA; Research; Research Design; Research Project Grants; Resistance; Resolution; Resource Sharing; Resources; Sampling; Services; Speed; Stains; Standardization; Stromal Cells; Structure; Supervision; Surveys; Technology; Testing; Tissues; Treatment outcome; United States National Institutes of Health; Universities; Validation; Visualization; Washington; Work; biomarker identification; cancer genome; clinical imaging; cost; exome sequencing; genome sequencing; improved; in vivo; individual response; molecular imaging; multiple omics; neoplastic cell; new technology; patient derived xenograft model; patient engagement; phosphoproteomics; potential biomarker; pre-clinical; programs; radiological imaging; senescence; single nucleus RNA-sequencing; stress reduction; targeted imaging; therapy resistant; tool; trait; transcriptome; transcriptome sequencing; transcriptomics; treatment response; tumor; tumor growth

SHARED RESOURCES CORE: SUMMARY The Shared Resources Core (SRC) is structured as a physical shared resource that will support both Research Projects, the PDX Core, as well as pilot and trans-Network projects. The most critical goal of the SRC is to provide services and resources that are either unavailable to individual research teams within PDXNet or that are unable to meet the cost, technical, and speed requirements of the Projects and other components at WU- PDTC. Other important missions for the SRC include optimization of the procedures for project/sample-specific requirements, standardization of the assays to ensure data consistency, and incorporation of the latest technologies. This centralized arrangement takes full advantage of the expertise and infrastructure existing at our institute developed through participation in several large-scale NIH consortia, including HTAN (Human Tumor Atlas Network), SenNet (Senescence Network), GUDMAP (GenitoUrinary Development Molecular Anatomy Project), CPTAC (Clinical Proteomic Tumor Analysis Consortium), PE-CGS (Participant Engagement and Cancer Genome Sequencing), CRIP (Co-Clinical Imaging Research Program), and PDXNet, in order to increase effectiveness and efficiency, avoid duplicated effort, and reduce stress on individual projects. SRC will be tightly integrated with the Research Projects, Pilot and Trans-Network Projects, the PDX Core, and the Bioinformatics Core under the supervision of the Administrative Core. Detailed characterization of the PDX tumors at the DNA, RNA, and protein levels will be important for validation, model selection, revelation of molecular changes in response to treatment, illustration of mechanisms of treatment resistance, as well as the identification of biomarkers and targets for future treatment testing. To accomplish these goals, the SRC will work with the Research Projects and other Cores to perform two main types of assays: Omics and imaging. For omics, the SRC will cover bulk and single cell omics, including whole exome sequencing (WES), bulk RNA-seq, single nucleus RNA-seq and ATAC-seq (snRNA-seq and snATAC-seq), spatial transcriptomics (Visium), as well as global proteomics and phospho-proteomics. For imaging, the SRC covers multiplex target imaging (CODEX/Phenocycler), in vivo preclinical molecular and radiologic imaging, and standard immunofluorescence (IF) staining. The SRC is also tasked with adjusting to the changing needs of the projects as well as introducing new technologies and tools to assist the Projects and to accomplish the mission of the center.

共享资源核心：摘要 共享资源核心（SRC）的结构是一个物理共享资源，将支持两个研究 项目，PDX核心，以及试点和跨网络项目。SRC最重要的目标是 提供PDXNet内的单个研究团队无法获得的服务和资源， 无法满足WU项目和其他组件的成本、技术和速度要求， PDTC。SRC的其他重要任务包括优化项目/样本特定的程序 要求，测定的标准化以确保数据一致性，并纳入最新的 技术.这种集中安排充分利用了现有的专门知识和基础设施， 我们的研究所通过参与几个大型NIH联盟而发展起来，包括HTAN（人类肿瘤 Atlas网络）、SenNet（衰老网络）、GUDMAP（生殖泌尿发育分子解剖学 项目），CPTAC（临床蛋白质组肿瘤分析联盟），PE-CGS（参与者参与和 癌症基因组测序），CRIP（联合临床成像研究计划）和PDXNet，以增加 有效性和效率，避免重复工作，并减少对单个项目压力。SRC将紧紧 与研究项目，试点和跨网络项目，PDX核心和生物信息学相结合 核心在行政核心的监督下。PDX肿瘤在DNA上的详细表征， RNA和蛋白质水平将是重要的验证，模型选择，揭示分子变化， 对治疗的反应，说明治疗抗性的机制，以及识别 生物标志物和未来治疗测试的目标。为了实现这些目标，SRC将与 研究项目和其他核心执行两种主要类型的测定：组学和成像。对于omics， SRC将涵盖批量和单细胞组学，包括全外显子组测序（WES），批量RNA-seq，单 核RNA-seq和ATAC-seq（snRNA-seq和snATAC-seq），空间转录组学（Visium），以及 全球蛋白质组学和磷酸化蛋白质组学。对于成像，SRC涵盖多目标成像 （CODEX/Phenocycler）、体内临床前分子和放射成像以及标准免疫荧光 (IF)染色该委员会亦负责因应工程项目不断转变的需要， 新的技术和工具，以协助项目和完成中心的使命。