NHP MHC COMPLEX ALLELE DISCOVERY AND TYPING TECHNOLOGY DEVELOPMENT - HIV/AIDS

“NHP MHC基因发现和分型开发项目的目标是推进非人灵长类动物（NHP）主要组织相容性复合体（MHC）遗传位点、等位基因和单倍型及其频率的详细知识库；扩大NHP杀伤细胞免疫球蛋白样受体（KIRs）基因、等位基因、单倍型和频率的发现和表征；并开发强大的、高通量的基因分型和单倍分型方法。”

“The goals of the NHP MHC Gene Discovery and Typing Development Program are to advance the detailed knowledge base of nonhuman primate (NHP) major histocompatibility complex (MHC) genetic loci, alleles, and haplotypes, and their frequencies; expand discovery and characterization of NHP killer-cell immunoglobulin-like receptors (KIRs) genes, alleles, haplotypes, and frequencies; and develop robust, high-throughput genotyping and haplotyping methods.” Currently the Indian rhesus macaque is one of the most widely used models for studying a variety of human health issues, ranging from diseases and disorders to potential therapies and preventive strategies. It is currently the best animal model for HIV vaccine testing, with the recent success of the RhCMV/SIV vaccination strategy contributing a most convincing example. Since cell-mediated immune responses to SIV are apparently crucial for this (and other) vaccine protection, a complete knowledge of the genetics of the immune response in macaques is essential for translation of results from the animal model to humans. This project will establish phased genomic sequences from the MHC class I and II regions and from KIR for rhesus macaques from the macaque SIV vaccine trial cohort tested at the Vaccine and Gene Therapy Institute at the Oregon Health & Sciences University; and establish phased genomic sequences from the Fc gamma receptor (FCGR) gene complex in 3 macaque species.