权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Vaccine Induced Activation of T Follicular Helper Cell Subsets

疫苗诱导滤泡辅助 T 细胞亚群的激活

基本信息

批准号：
8307073
负责人：
Hideki Ueno
金额：
$ 20.89万
依托单位：
BAYLOR RESEARCH INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-07-05 至 2015-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8307073
关键词：
Ablation Adult Antibody Formation Antigens B-Lymphocytes BLR1 gene Biological Assay Biological Markers Blood Blood specimen CD14 gene CD4 Positive T Lymphocytes Cells Cellular Immunity Cellular Structures Characteristics Defect Dendritic Cells Dermal Development Disease Enhancing Antibodies Equilibrium Frequencies Future Goals Helper-Inducer T-Lymphocyte Human Humoral Immunities Immune Immune system Immunity Immunoglobulin Class Switching Immunoglobulin-Secreting Cells Individual Influenza vaccination Instruction Interleukin-12 Langerhans cell Lymphoid Memory MicroRNAs Modeling Molecular Profiling Myelogenous Natural Immunity Organ Outcome Patients Pattern Phenotype Play Property RNA Role ST14 gene Sorting - Cell Movement Structure of germinal center of lymph node Surface Systems Analysis T cell response T-Lymphocyte T-Lymphocyte Subsets Testing Time Transcript Vaccinated Vaccination Vaccine Design Vaccines adaptive immunity arm cell growth chemokine receptor cohort cytokine design flu improved in vitro Assay in vivo influenza virus vaccine insight novel novel marker receptor expression response seasonal influenza tool

项目摘要

Innate and adaptive immunity are both activated upon vaccination. We have recently demonstrated that the two adaptive immune arms (cellular and humoral immunity) are uniquely induced by different types of myeloid dendritic cell (DC) subsets. In particular, IL-12 induces the development of CD4+ T cells capable of helping B cells through the secretion of IL-21, a cytokine that potently promotes B cell growth, differentiation, and classswitching. These CD4-I- T cells share properties with T follicular helper cells (Tfh), a recently established specialized B cell-helper CD4-f T cell subset. Human Tfh cells can be found in the germinal centers of secondary lymphoid organs and in blood, and are characterized by the expression of the chemokine receptor CXCRS and the secretion of IL-21. Thus, Tfh cells appear to playa fundamental role in the developmentof antibody responses ¿ upon vaccination. However, little is known about how vaccines induce Tfh responses and how the induced Tfh cells regulate antibody responses. Our recent studies indicate that Tfh cells from human blood are composed of functionally distinct subsets. The main hypothesis of this project is that the differential mobilization of Tfh subsets dictates the quality and quantity of vaccine-induced humoral immunity. In particular, we hypothesize that a positive vaccine outcome is associated with activation of helper Tfh cells (i.e., Tfh2 and Tfhl7 cells). In contrast, a negative vaccine outcome is associated with either a defect of helper Tfh cell activation or an overactivation of suppressor Tfh cells, i.e., Tfhl cells. In this Project, we will establish the molecular signatures of blood Tfh cells and their functionally different components at baseline, and upon activation following Flu vaccination. The ultimate goal of this Project is to generate tools that will permit high fidelity assessment of in vivo activation of Tfh subsets and prediction of protective responses to vaccines.

接种疫苗后，先天免疫和适应性免疫都会被激活。我们最近证明了这两点