Core Grant for Vision Research - Core #1 Gene Delivery and Quantitative Molecular Biology

视觉研究核心资助 - 核心

• 批准号: 10000152
• 负责人: ROBERT W NICKELLS
• 金额: $ 18.12万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2021-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10000152
• 关键词: Alleles; Biotechnology; Consultations; Core Grant; Data; Data Analyses; Doctor of Philosophy; Equipment; Gene Delivery; Goals; Grant; In Situ Hybridization; Individual; Molecular Biology; Mus; Nucleic Acid Probes; Plasmid Cloning Vector; Proteomics; Qualifying; Reaction; Reporter Genes; Research Personnel; Resources; Services; Training; Viral Vector; Vision; Vision research; design; next generation sequencing; vector

CORE 1: VECTOR AND GENE DELIVERY/QUANTITATIVE MOLECULAR BIOLOGY ABSTRACT The goal of Core 1 is to provide the following services to users with qualifying grants and other vision researchers. The services include consultation on the use of gene delivery vectors, construction of plasmid and viral vectors, packaging and validating viral vectors, training individuals to construct and package viral vectors, providing consultation for quantitative PCR, providing access to qPCR equipment, carrying out qPCR reactions for users, providing qPCR data analysis, allelic characterization of normal and genetically modified mice, nucleic acid probe design, in situ hybridization, quantitative reporter gene analysis, and to facilitate use of campus resources such as the Biotechnology Center (NextGen Sequencing, data handling and analysis, proteomics, etc.).

核心1：载体和基因递送/定量分子生物学 摘要 Core 1的目标是向具有合格赠款和其他愿景的用户提供以下服务 研究人员服务内容包括基因传递载体的使用咨询、质粒构建咨询、 包装和验证病毒载体，培训个人构建和包装病毒载体， 载体，提供定量PCR咨询，提供qPCR设备，进行qPCR 反应，提供qPCR数据分析，正常和转基因的等位基因表征 小鼠，核酸探针设计，原位杂交，定量报告基因分析，并方便使用 校园资源，如生物技术中心（下一代测序，数据处理和分析， 蛋白质组学等）。