High-Throughput Platform for Thermodynamic Characterization of Biomolecular Interactions

用于生物分子相互作用热力学表征的高通量平台

• 批准号: 10021436
• 负责人: Joerg Martini
• 金额: $ 74.59万
• 依托单位: PALO ALTO RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-20 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10021436
• 关键词: Address; Adoption; Automation; Biochemical; Biochemical Reaction; CCL18 gene; Calorimetry; Color; Communities; Consumption; Data; Data Analyses; Databases; Detection; Development; Encapsulated; Gelatin; Genomics; Goals; Heating; Hour; Immobilization; Individual; Label; Lasers; Libraries; Ligand Binding; Ligands; Lighting; Liquid substance; Measurement; Microfluidic Microchips; Microfluidics; Names; Oils; Optics; Organelles; Peptide antibodies; Performance; Phase; Proteins; Proteomics; Reaction; Reagent; Research; Resolution; Sampling; Screening procedure; System; Techniques; Technology; Temperature; Testing; Thermodynamics; Time; Titrations; Travel; Validation; Water; Work; aqueous; base; biological systems; data analysis pipeline; enthalpy; high throughput screening; improved; inhibitor/antagonist; instrument; interest; liquid crystal; miniaturize; nanolitre; particle; protein function; prototype; receptor; small molecule

In this project we plan to further develop our microfluidic droplet calorimetry platform to enable thermodynamic characterization of a wide variety and large number of biomolecular interactions. Having shown proof of concept of the technology, we plan to increase the sensitivity and throughput of the system to enable characterization of a broad range of reactions. The aims of the project are to 1) achieve multiplexed high- resolution temperature readout for individual droplets, 2) increase the throughput of the system via automation of the fluidic handling and data analysis, and 3) demonstrate and validate the technique by performing calorimetric measurements of a variety of test samples. Taken together, these improvements will allow for a dramatic expansion of calorimetry’s capabilities and, further, make those capabilities more accessible to the scientific community.

在这个项目中，我们计划进一步开发我们的微流控液滴热计平台，以实现热力学 描述种类繁多、数量众多的生物分子相互作用。已出示证据证明 这项技术的概念，我们计划增加系统的灵敏度和吞吐量，以使 描述了一系列反应的特征。该项目的目标是1)实现多路高性能 单个液滴的分辨率温度读数，2)通过自动化提高系统的吞吐量 流体处理和数据分析，以及3)通过执行 各种测试样品的量热测量。总而言之，这些改进将允许 量热仪功能的戏剧性扩展，进一步使这些功能更容易为 科学界。