Imaging mass spectrometry at isomeric chemical resolution using gas phase ion/ion reactions
使用气相离子/离子反应进行异构化学分辨率成像质谱分析
基本信息
- 批准号:10027319
- 负责人:
- 金额:$ 30.48万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-01 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAffectAminesAmino AcidsAntineoplastic AgentsBiochemicalBiochemical PathwayBiochemical ProcessBiologicalBiological ProcessBiomedical ResearchCell physiologyCellsChemical StructureChemicalsChemistryCholineClinicalCommunicable DiseasesComplex MixturesDetectionDevelopmentDiabetes MellitusFailureFatty AcidsFunctional disorderGasesGenerationsGoalsHeadImageIn SituIndividualIonsKineticsLabelLecithinLipidsMalignant NeoplasmsMapsMass Spectrum AnalysisMeasurementMethodologyMethodsModificationMolecularPeptidesPerformancePharmaceutical PreparationsPharmacologyPhasePhosphatidylethanolaminePhosphatidylserinesPlayPositioning AttributeProteomicsReactionReagentReproducibilityResolutionResourcesRoleSamplingScanningSeriesSignal TransductionSiteSpatial DistributionSpecificitySpecimenSpeedSphingomyelinsStructureSurfaceTechnologyTimeTissue SampleTissue imagingTissuesVisualizationbasedesigndetectorexperimental studyflexibilityfunctional groupimaging approachimaging modalityimprovedinstrumentinstrumentationinterestmass spectrometermetabolomicsmicroscopic imagingmolecular imagingmolecular massnovelpi bondpreventsmall moleculetandem mass spectrometrytooltreatment strategy
项目摘要
PROJECT SUMMARY
Molecular imaging plays a pivotal role in biomedical research. By enabling the visualization of biological
processes directly in tissue, in situ assessments of cellular function can be recorded with spatial context. The
use of mass spectrometry as a molecular imaging modality combines the high level of molecular specificity
provided by the mass spectrometer with the spatial fidelity of a microscopic imaging approach. By this, imaging
mass spectrometry (IMS) provides for the label-free mapping of a wide array of biomolecules in tissue
specimens. Accurate identification of the biochemical pathways altered during development and dysfunction is
a key step in designing novel treatment strategies for a variety of applications, such as in studies of diabetes,
infectious disease, drug pharmacology, and cancer. However, severe deficiencies remain in the differentiation
and structural identification of molecules detected during imaging mass spectrometry experiments due to the
enormous chemical complexity of tissue samples. The failure to adequately separate and identify these
compounds results in ion images consisting of multiple different compounds with overlapping masses. This
distorted picture of molecular distributions clouds the interpretation of the biochemical maps produced by imaging
mass spectrometry and prevents a complete and accurate understanding of cellular compositions and functions.
This proposal aims to develop methods and instrumentation that will enable tissue imaging at unparalleled levels
of sensitivity, separation, and identification. This will be achieved through the discovery and development of
novel gas-phase ion/ion reactions that target specific chemical functional groups in lipids and metabolites
(Specific Aim 1). These reactions offer rapid and flexible means for molecular transformations without
manipulating the tissue sample and can result in improved detection limits and more extensive chemical
structural information. Developing reproducible and quantitative ion/ion reaction methodologies will enable
reliable measurements to be made from tissue (Specific Aim 2). The development of instrumentation that can
perform gas-phase ion/ion reactions with high throughput will enable these transformations to be performed
directly during imaging mass spectrometry experiments (Specific Aim 3). These ‘reactive’ images are anticipated
to reveal spatial biochemical detail unobtainable by conventional imaging modalities. The continual development
of new analytical technologies such as those proposed herein is crucial in order to address increasingly
complicated biological and clinical questions.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Boone M. Prentice其他文献
Spatial mapping of phosphatidylcholine <em>sn</em>-positional isomers using CID of divalent metal complexes in imaging mass spectrometry
- DOI:
10.1016/j.ijms.2024.117370 - 发表时间:
2025-02-01 - 期刊:
- 影响因子:
- 作者:
Tingting Yan;Zunaira Naeem;Zhongling Liang;Hassan Azari;Brent A. Reynolds;Boone M. Prentice - 通讯作者:
Boone M. Prentice
Formation of multiple ion types during MALDI imaging mass spectrometry analysis of emMitragyna speciosa/em alkaloids in dosed rat brain tissue
在对给药大鼠脑组织中的帽蕊木生物碱进行基质辅助激光解吸电离成像质谱分析过程中多种离子类型的形成
- DOI:
10.1016/j.talanta.2024.125923 - 发表时间:
2024-07-01 - 期刊:
- 影响因子:6.100
- 作者:
Zhongling Liang;Yingchan Guo;Nicholas Ellin;Tamara I. King;Erin C. Berthold;Sushobhan Mukhopadhyay;Abhisheak Sharma;Christopher R. McCurdy;Boone M. Prentice - 通讯作者:
Boone M. Prentice
Spatial mapping of the brain metabolome lipidome and glycome
大脑代谢组、脂质组和糖组的空间映射
- DOI:
10.1038/s41467-025-59487-7 - 发表时间:
2025-05-12 - 期刊:
- 影响因子:15.700
- 作者:
Harrison A. Clarke;Xin Ma;Cameron J. Shedlock;Terrymar Medina;Tara R. Hawkinson;Lei Wu;Roberto A. Ribas;Shannon Keohane;Sakthivel Ravi;Jennifer L. Bizon;Sara N. Burke;Jose Francisco Abisambra;Matthew E. Merritt;Boone M. Prentice;Craig W. Vander Kooi;Matthew S. Gentry;Li Chen;Ramon C. Sun - 通讯作者:
Ramon C. Sun
Liberation of host heme by emClostridioides difficile-/emmediated damage enhances emEnterococcus faecalis/em fitness during infection
艰难梭菌介导的宿主血红素释放损伤增强了粪肠球菌在感染期间的适应性
- DOI:
10.1128/mbio.01656-23 - 发表时间:
2023-12-11 - 期刊:
- 影响因子:4.700
- 作者:
Alexander B. Smith;Jonathan T. Specker;Katharine K. Hewlett;Troy R. Scoggins;Montana Knight;Abigail M. Lustig;Yanhong Li;Kirsten M. Evans;Yingchan Guo;Qianxuan She;Michael W. Christopher;Timothy J. Garrett;Ahmed M. Moustafa;Daria Van Tyne;Boone M. Prentice;Joseph P. Zackular;Kimberly A. Kline - 通讯作者:
Kimberly A. Kline
A mass spectrometry–based assay for mouse IgG N-glycan screening in biofluids
- DOI:
10.1007/s00216-025-05994-x - 发表时间:
2025-07-07 - 期刊:
- 影响因子:3.800
- 作者:
Ariana E. Stratton;Hassan Moussa;Yingchan Guo;Justin M. Ellenburg;Carl Atkinson;Boone M. Prentice - 通讯作者:
Boone M. Prentice
Boone M. Prentice的其他文献
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{{ truncateString('Boone M. Prentice', 18)}}的其他基金
Administrative Supplements to Support Undergraduate Summer Research Experiences
支持本科生暑期研究经历的行政补充
- 批准号:
10393320 - 财政年份:2020
- 资助金额:
$ 30.48万 - 项目类别:
Imaging mass spectrometry at isomeric chemical resolution using gas phase ion/ion reactions
使用气相离子/离子反应进行异构化学分辨率成像质谱分析
- 批准号:
10246507 - 财政年份:2020
- 资助金额:
$ 30.48万 - 项目类别:
Imaging mass spectrometry at isomeric chemical resolution using gas phase ion/ion reactions
使用气相离子/离子反应进行异构化学分辨率成像质谱分析
- 批准号:
10418780 - 财政年份:2020
- 资助金额:
$ 30.48万 - 项目类别:
Imaging mass spectrometry at isomeric chemical resolution using gas phase ion/ion reactions
使用气相离子/离子反应进行异构化学分辨率成像质谱分析
- 批准号:
10669048 - 财政年份:2020
- 资助金额:
$ 30.48万 - 项目类别:
Molecular Profiling of Pancreatic Pathophysiology by Imaging Mass Spectrometry
通过成像质谱法对胰腺病理生理学进行分子分析
- 批准号:
8908974 - 财政年份:2015
- 资助金额:
$ 30.48万 - 项目类别:
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