Craniofacial Morphogenesis in Prenatal Alcohol Exposure

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ABSTRACT Prenatal alcohol exposure is a leading cause of neurodevelopmental disability and may be accompanied by characteristic facial deficits caused, in part, by the apoptotic deletion of neural crest progenitor cells. The parent R01 (AA011085) tests the hypothesis that ribosome dysbiogenesis and nucleolar stress drive this apoptosis, based on our whole transcriptome analysis of these cells. Exon-level reanalysis of that dataset uncovered 19 miRNAs having altered representation in response to alcohol. One of these, miR- 3064-3p, is induced by alcohol (Fold-change 1.55, p=0.0002). miR-3064 is conserved across vertebrates and is implicated in our ribosome dysbiogenesis and calcium-mediated apoptosis. Dr. Olivia Rivera joined my lab July 22, 2019 to obtain training in epigenetics, and how alcohol dysregulates epigenetic signals to impede embryo development. Her Ph.D. identified a polymorphism in a zinc transporter that affects lactation in human and mouse. Upon discussion, we determined that investigation into miR- 3064’s role in neural crest apoptosis addresses this training, represents a logical extension of the parent award, and positions Dr. Rivera for an independent career in alcohol and epigenetics.