Super-resolution imaging of the ocular fundus

眼底超分辨率成像

• 批准号: 10002236
• 负责人: Kristina IRSCH
• 金额: $ 16.17万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10002236
• 关键词: Acceleration; Address; Adult; Age related macular degeneration; Algorithms; Automobile Driving; Blindness; Brain; Cell Death; Cells; Cellular Structures; Clinical; Complex; Detection; Development; Device or Instrument Development; Diabetic Retinopathy; Diagnosis; Disease; Early Diagnosis; Environment; Eye; Eye Movements; Floods; Fundus; Fundus photography; Future; Glaucoma; Human; Human Volunteers; Hypertension; Image; Imaging technology; Individual; Innovative Therapy; Institutes; Investigation; Knowledge; Lasers; Lateral; Light; Lighting; Measurement; Measures; Methods; Microscopy; Morphologic artifacts; Motion; Movement; Multiple Sclerosis; Neurosciences; Noise; Ophthalmology; Optical Coherence Tomography; Optics; Pathogenesis; Pathway interactions; Pattern; Positioning Attribute; Process; Psychological Techniques; Public Health; Pupil; Research Personnel; Resolution; Retina; Retinal Diseases; Retinal Dystrophy; Running; Safety; Scanning; Scheme; Sclera; Signal Transduction; Structure; Subcellular structure; System; Techniques; Technology; Time; Validation; Variant; Vision; Visual system structure; adaptive optics; adaptive optics scanning laser ophthalmoscopy; base; blind; data acquisition; design; empowered; experimental study; fundus imaging; image reconstruction; image registration; imaging capabilities; imaging modality; improved; in vivo; in vivo imaging; insight; interest; novel; novel strategies; ocular imaging; reconstruction; retinal imaging; sample fixation; submicron; therapeutic development

Project Summary Remarkable developments in ophthalmic imaging technologies, including optical coherence tomography (OCT) and more recently adaptive-optics retinal imaging have enabled micron-level resolution and transformed diagnosis and management of ophthalmic diseases. Unfortunately, still many of the retinal structures cannot be resolved in vivo, let alone sub-cellular structures. A key shortcoming shared by existing optical approaches for retinal imaging is that the attainable size of the eye’s pupil sets a limit on the numerical aperture, and hence the lateral resolution. At the same time, in many cases, illumination and detection pathways have to be separated within this sole aperture of the eye, further constraining the achievable resolution. In addition, contrast variations among the structures of interest, noise of the overall system, and eye motion artifacts further limit current approaches. Time is ripe for another ophthalmic imaging revolution that overcomes existing constraints and enables the uncovering of retinal structures at the cellular and sub-cellular level in the living eye. The objective of this project is to address this unmet need by exploring speckle structured-illumination of the ocular fundus. Furthermore, the project will investigate laser Illumination through the sclera to overcome the numerical- aperture limitations inherent to pupil-based illumination. The proposed approach allows the formation of speckle patterns on the ocular fundus that are randomly shifted (scanned) by the naturally occurring involuntary fixational movements of the eye. The backscattered light is collected through the pupil and post-processed to recover super-resolved retinal images, beyond the capabilities of state-of-the art imaging modalities. Interestingly, the eye point spread function is obtained as a by-product describing the aberrations of eye. Movement artifacts are avoided by collecting short exposure image frames which are carefully registered before reconstruction. An eye movement sensing subsystem enables sub-diffraction precision registration. The investigators have assembled the required multifaceted expertise to explore, for the first time, these bold ideas. It includes leaders in random media, super-resolution, and ophthalmic instrument development. At the end of this project, we expect to be in a unique position to transfer the technique into practice through the team’s existing wide cooperative network of ophthalmology departments. Armed with the unprecedented spatial resolution of the proposed technique, these future interdisciplinary investigations can drive new insights into retinal disease mechanisms and enable earlier diagnosis of retinal diseases, including some of the most severe vision disabling conditions (e.g., retinal dystrophies, age-related macular degeneration, glaucoma), while driving innovative therapies to protect cells before irreversible cell death and blindness occurs.

项目概要 眼科成像技术的显着发展，包括光学相干断层扫描 (OCT) 最近，自适应光学视网膜成像已经实现了微米级分辨率，并改变了 眼科疾病的诊断和治疗。不幸的是，仍然有许多视网膜结构无法被 体内已经解决了，更不用说亚细胞结构了。 现有视网膜成像光学方法共有的一个主要缺点是，可达到的尺寸 眼睛的瞳孔限制了数值孔径，从而限制了横向分辨率。与此同时，在许多 在这种情况下，照明和检测路径必须在眼睛的唯一孔径内分开，进一步 限制了可实现的分辨率。此外，感兴趣的结构之间的对比度变化、噪声 整个系统和眼动伪影进一步限制了当前的方法。 另一场眼科成像革命的时机已经成熟，该革命将克服现有的限制并实现 在活体眼睛的细胞和亚细胞水平上揭示视网膜结构。此举的目的 该项目旨在通过探索眼底的散斑结构照明来解决这一未满足的需求。 此外，该项目将研究通过巩膜的激光照明，以克服数值- 基于光瞳的照明固有的孔径限制。 所提出的方法允许在眼底上形成随机移动的散斑图案 （扫描）通过眼睛自然发生的不自主注视运动。后向散射光为 通过瞳孔收集并进行后处理以恢复超分辨率的视网膜图像，超出了 最先进的成像模式的能力。有趣的是，眼点扩散函数是作为 描述眼睛像差的副产品。通过收集短曝光来避免运动伪影 在重建之前仔细配准的图像帧。眼球运动传感子系统 实现亚衍射精确配准。 研究人员汇集了所需的多方面专业知识，首次探索这些大胆的 想法。它包括随机媒体、超分辨率和眼科仪器开发领域的领导者。 在该项目结束时，我们希望能够处于独特的地位，通过以下方式将该技术转化为实践： 该团队现有广泛的眼科合作网络。配备前所未有的 所提出技术的空间分辨率，这些未来的跨学科研究可以带来新的见解 研究视网膜疾病机制并能够早期诊断视网膜疾病，包括一些最常见的疾病 严重视力障碍（例如视网膜营养不良、年龄相关性黄斑变性、青光眼）， 同时推动创新疗法，在不可逆转的细胞死亡和失明发生之前保护细胞。