Noise, memory, and adaptation in the flagellum system in E.coli.

大肠杆菌鞭毛系统的噪音、记忆和适应。

• 批准号: 10004140
• 负责人: Philippe Cluzel
• 金额: $ 32.12万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10004140
• 关键词: Affect; Automobile Driving; Bacteria; Biological Assay; Biological Models; Biology; Catheters; Cells; Data; Environment; Escherichia coli; Exhibits; Filament; Fluorescence Microscopy; Generations; Genes; Goals; Growth; Health Hazards; Individual; Knowledge; Masks; Medical; Medical Device; Memory; Microbial Biofilms; Microfluidics; Monitor; Noise; Nutrient; Organelles; Physiologic pulse; Population Study; Production; Proteins; Regulator Genes; Resolution; Sigma Factor; System; Techniques; Text; Time; cell growth; cell type; cost; design; fitness; response

Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. The long-term goal of this project is to identify and predict the activity of gene regulatory networks (GRN) of highly expressed genes when bacteria need to grow in nutrient-limited environments. To quantitively tackle this problem, we will use as a model system the flagellum GRN in E. coli. While a lot is known about the flagellum GRN, most of our information comes from population studies that mask the regulatory dynamics taking place within uncoordinated single cells. Consequently, this project will make use of well-known fluorescence microscopy techniques and more recent advances in microfluidics to monitor at high-resolution growth and the activity of the flagellum GRN in single cells under different environmental conditions. We have organized this proposal around one of the most intriguing aspect of our preliminary data that demonstrates that the flagellum GRN exhibits pulsating dynamics. We found that the expression of flagellar genes in wild-type cells is either ‘off’ over several generations or is ‘on’ for a much shorter period of time. We propose to determine the growth cost of single pulses in individual cells. This aim will help us to identify how modulating the dynamics of the flagellum GRN activity can be a strategy to optimize both cellular growth and the synthesis of large organelles under nutrient limited conditions. At the completion of this proposal our current knowledge on the flagellum synthesis under nutrient limited conditions will be significantly advanced and we hope that some of the newly identified principles could be used in quantitative biology to optimize the designs of synthetic circuits in fluctuating nutrient-poor environments.

在此处输入文本，这是您应用程序的新摘要信息。本节必须不超过30行文本。 该项目的长期目标是识别和预测高度表达基因基因调节网络（GRN）的活性，而细菌需要在营养限制的环境中生长。为了定量解决此问题，我们将用作模型系统。 尽管对鞭毛GRN有很多了解，但我们的大多数信息都来自掩盖不协调单细胞内发生的调节动态的人群研究。因此，该项目将利用众所周知的荧光显微镜技术和微流体的最新进展，以在不同环境条件下在单个细胞中以高分辨率生长和鞭毛GRN的活性进行监测。我们围绕了初步数据中最有趣的方面之一，这表明Flagelum GRN表现出脉动动力学。我们发现，野生型细胞中鞭毛基因的表达要么在几代人中是“关闭”的，要么“ ON”在短时间内。我们建议确定单个细胞中单个脉冲的增长成本。这个目标将有助于我们确定如何调节鞭毛GRN活性的动力学是如何在养分有限条件下优化细胞生长和大型细胞器的合成的一种策略。 该提案完成后，我们目前在养分有限条件下对鞭毛合成的知识将得到显着提高，我们希望可以在定量生物学中使用一些新确定的原理，以优化在自动贫乏环境中的合成回路设计。