Retinal Mechanisms

项目总结/摘要 由Jennifer Hunter和大卫威廉姆斯领导的罗切斯特大学的一个高度合作的团队将在体内将双光子激发荧光检眼镜（TPEFO）从猴子翻译到人类。该技术有可能提供关于显微视网膜形态学的新信息，并作为评估视网膜功能的上级、客观的措施，这是现有成像技术中罕见的能力。IR自发荧光减少的影响，目前唯一值得注意的，TPEFO的后果将被表征。此外，我们将建立视网膜损伤的阈值。在重复TPEF成像后监测皮质盲人类受试者的视网膜健康将确定TPEFO是否可以安全地应用于正常人眼。

PROJECT SUMMARY/ABSTRACT A highly collaborative team at the University of Rochester led by Jennifer Hunter and David Williams will translate in vivo two-photon excited fluorescence ophthalmoscopy (TPEFO) from monkey to human. This technology has the potential to provide new information on microscopic retinal morphology and to serve as a superior, objective measure to assess retinal function, a rare capability among existing imaging technologies. Effects of IR autofluorescence reduction, currently the only noticeable, consequence of TPEFO will be characterized. Additionally, we will establish thresholds for retinal damage. Monitoring retinal health in cortically blind human subjects after repeated TPEF imaging will establish whether TPEFO can be applied safely to normal human eyes.