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High-Resolution Functional Imaging of the Retina

视网膜的高分辨率功能成像

基本信息

批准号：
9554180
负责人：
Jennifer J Hunter
金额：
$ 4.36万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-04-01 至 2018-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9554180
关键词：
Address Affect Aging All-Trans-Retinol Back Biochemical Biochemical Pathway Biochemical Process Biology Cell physiology Cells Collection Cone Dark Adaptation Degenerative Disorder Development Diagnosis Disease Engineering Esters Eye Eye diseases Financial compensation Fluorescence Functional Imaging Glaucoma Goals Human Image Individual Knowledge Laboratories Lasers Lead Leber&apos s Hereditary Optic Neuropathy Light Macaca Macular degeneration Measurement Measures Metabolism Methodology Methods Microscope Microscopy Molecular Monitor Monkeys Mosaicism Motion Mus NADH Natural regeneration Neurons Normal Cell Ophthalmoscopes Ophthalmoscopy Optics Penetration Photic Stimulation Photons Photoreceptors Physiologic pulse Process Radiation Research Resolution Rest Retina Retinal Retinal Diseases Scanning Source Stargardt&apos s disease Structure Techniques Technology Time Tissues Treatment Efficacy Universities Vertebrate Photoreceptors Visible Radiation Vision Work absorption adaptive optics contrast imaging drug efficacy experimental study falls fluorescence imaging fluorophore ganglion cell imaging modality imaging system improved in vivo insight microscopic imaging mitochondrial metabolism molecular dynamics optical spectra response treatment strategy two-photon visual cycle

项目摘要

Project Summary We wish to understand two important processes that sustain human vision; the visual cycle is responsible for regeneration of photopigment bleached by absorption of visible light and cellular metabolism is required in every living cell to provide energy. To study these processes, we need a method to visualize individual cells and measure molecular dynamics in the living eye. Some of the molecules involved are intrinsically fluorescent, but are inaccessible in the living eye with single- photon fluorescence imaging because the excitation falls outside the range of radiation that can penetrate the optics of the eye. By using considerably longer excitation wavelengths, two-photon excited fluorescence imaging has the potential to excite these otherwise inaccessible fluorophores and provide intrinsic contrast for imaging a number of retinal structures. In our initial experiments, we used an adaptive optics scanning light ophthalmoscope (AOSLO) to image two-photon fluorescence from cone inner segments in the living macaque eye (Hunter et al, 2011). By correcting the longitudinal chromatic aberration and material dispersion of the eye, we plan to improve the efficiency of our imaging systems and develop methodology for reliably imaging both structure and function of multiple retinal layers in the eye. Not only will this capability provide insight into normal cell mosaics and their biochemical processes, it will also improve our understanding of many diseases that impact these retinal biochemical cascades such as Stargardt's disease, macular degeneration and Leber's hereditary optic neuropathy.

项目摘要我们希望了解维持人类视觉的两个重要过程;视觉周期是负责通过吸收可见光和细胞色素而漂白的色素的再生每个活细胞都需要新陈代谢来提供能量。为了研究这些过程，我们需要一个方法来可视化单个细胞和测量分子动力学在活的眼睛。一些所涉及的分子本质上是荧光的，但在活体眼睛中是无法用单- 光子荧光成像，因为激发福尔斯落在可以穿透眼睛的光学系统。通过使用相当长的激发波长，激发荧光成像具有激发这些否则难以接近的荧光团的潜力并提供用于成像许多视网膜结构的固有对比度。在最初的实验中，我们使用自适应光学扫描光检眼镜（AOSLO）对双光子荧光成像来自活猕猴眼睛中的视锥内节（Hunter等人，2011）。通过校正纵向色差和材料色散的眼睛，我们计划提高效率我们的成像系统和开发方法，可靠地成像的结构和功能，眼睛里的多层视网膜。这种能力不仅可以提供对正常细胞镶嵌的深入了解，以及它们的生化过程，它也将提高我们对许多疾病的理解，这些视网膜生化级联反应，如Stargardt病，黄斑变性和Leber病，遗传性视神经病变