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High-Resolution Functional Imaging of the Retina

视网膜的高分辨率功能成像

基本信息

批准号：
9049503
负责人：
Jennifer J Hunter
金额：
$ 34.76万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-04-01 至 2017-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9049503
关键词：
Address Affect Aging All-Trans-Retinol Back Biochemical Biochemical Pathway Biochemical Process Biology Cell physiology Cells Collection Cone Dark Adaptation Degenerative Disorder Development Diagnosis Disease Engineering Esters Eye Financial compensation Fluorescence Functional Imaging Glaucoma Goals Human Image Individual Knowledge Laboratories Lasers Lead Leber&apos s Hereditary Optic Neuropathy Life Light Macaca Macular degeneration Measurement Measures Metabolism Methodology Methods Microscope Microscopy Molecular Monitor Monkeys Motion Mus NADH Natural regeneration Neurons Normal Cell Ophthalmoscopes Ophthalmoscopy Optics Penetration Pharmacotherapy Photic Stimulation Photons Photoreceptors Physiologic pulse Process Radiation Research Resolution Rest Retina Retinal Retinal Diseases Scanning Source Stargardt&apos s disease Structure Techniques Technology Time Tissues Universities Vertebrate Photoreceptors Visible Radiation Vision Work absorption adaptive optics contrast imaging falls fluorescence imaging fluorophore ganglion cell imaging modality imaging system improved in vivo insight microscopic imaging mitochondrial metabolism molecular dynamics optical spectra research study response treatment strategy two-photon visual cycle

项目摘要

Project Summary We wish to understand two important processes that sustain human vision; the visual cycle is responsible for regeneration of photopigment bleached by absorption of visible light and cellular metabolism is required in every living cell to provide energy. To study these processes, we need a method to visualize individual cells and measure molecular dynamics in the living eye. Some of the molecules involved are intrinsically fluorescent, but are inaccessible in the living eye with single- photon fluorescence imaging because the excitation falls outside the range of radiation that can penetrate the optics of the eye. By using considerably longer excitation wavelengths, two-photon excited fluorescence imaging has the potential to excite these otherwise inaccessible fluorophores and provide intrinsic contrast for imaging a number of retinal structures. In our initial experiments, we used an adaptive optics scanning light ophthalmoscope (AOSLO) to image two-photon fluorescence from cone inner segments in the living macaque eye (Hunter et al, 2011). By correcting the longitudinal chromatic aberration and material dispersion of the eye, we plan to improve the efficiency of our imaging systems and develop methodology for reliably imaging both structure and function of multiple retinal layers in the eye. Not only will this capability provide insight into normal cell mosaics and their biochemical processes, it will also improve our understanding of many diseases that impact these retinal biochemical cascades such as Stargardt's disease, macular degeneration and Leber's hereditary optic neuropathy.

项目总结