Mays Cancer Center at UT Health SA

UT Health SA 梅斯癌症中心

• 批准号: 10025091
• 负责人: Zhao Lai
• 金额: $ 8.47万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10025091
• 关键词: Advisory Committees; Applications Grants; Area; Base Sequence; Bioinformatics; Bioinformatics Shared Resource; Biological Assay; Biometry; CRISPR screen; Cancer Center; Cancer Center Support Grant; Catchment Area; Cells; ChIP-seq; Child; Chromosome Structures; Collaborations; Complement; Consultations; Copy Number Polymorphism; Custom; DNA Damage; DNA copy number; DNA sequencing; Data; Diagnosis; Doctor of Philosophy; Educational workshop; Environment; Equipment; Experimental Designs; Faculty; Funding; Funding Agency; Future; Gene Expression; Gene Expression Regulation; Generations; Genomics; Goals; Group Meetings; Health; Journals; Knowledge; Libraries; Link; Malignant Neoplasms; Manuscripts; Metagenomics; MicroRNAs; Mission; Mutation; Nature; Pathway interactions; Peer Review; Phenotype; Population; Preparation; Price; Protocols documentation; Publications; Reagent; Research; Research Institute; Research Personnel; Resource Sharing; Sampling; Screening for cancer; Services; Small RNA; Statistical Data Interpretation; Strategic Planning; Students; Technology; Training; Tumor Subtype; Tumor Suppressor Proteins; Variant; Work; anticancer research; cancer cell; cancer heterogeneity; cancer initiation; chromatin modification; cost effective; data pipeline; design; direct application; experimental study; genomic data; improved; liquid biopsy; member; next generation sequencing; novel; outcome forecast; programs; single cell analysis; square foot; tool; transcriptome sequencing; tumor heterogeneity

Project Summary—Next Generation Sequencing Shared Resource The mission of the Next-Generation Sequencing Shared Resource (NGSSR) is to provide Mays Cancer Center (MCC) members with state-of-the-art next generation sequencing technology for detecting and exploring alterations in nucleotide sequence, DNA copy number variation, gene expression differences, chromosome structure variation, chromatin modification and all interactions which are linked to the complexity of phenotypes seen in cancers. The NGSSR, directed by Zhao Lai, Ph.D., has been an MCC Shared Resource since 2012. It is located in 1,000 sq. ft. of space in the Greehey Children’s Cancer Research Institute (GCCRI) building, adjacent to the MCC. Dr. Lai is assisted by technical director Ms. Dawn Garcia. The NGSSR conducts sequence applications for MCC users across all 3 scientific programs. Services include various types of library construction, DNA-seq, RNA-seq, ChIP-seq, small RNA-seq and exomeCap-seq, CRISPR screening, 16S metagenomics, and single cell analysis. In addition, Dr. Lai and the NGSSR team assist users with experimental design and provide educational training to faculty, staff and students. The NGSSR works closely with the Biostatistics and Bioinformatics Shared Resource (BBSR), led by Yidong Chen, Ph.D. (PSP), a bioinformatics expert. The BBSR works with the NGSSR to provide MCC members with computational capabilities that complement the NGSSR’s offerings. The NGSSR is overseen by a Scientific Advisory Committee and the MCC Shared Resource Oversight Committee (SROC). In 2018, the NGSSR was used by 31 peer-reviewed funded MCC members (36% of total usage) and contributed to 12 cancer-related manuscripts in 2018. Since its inception, from 2014 to the middle of 2018, NGSSR has quickly become an essential component in MCC and transformed the genomics research environment for MCC members with a nearly three-fold increase in the number of users and contributions to over 44 cancer-related publications in NEJM, Nature, Cancer Cell, PNAS, and other high-impact peer-reviewed journals. As new tools and reagents are developed (e.g. liquid biopsies) they can be readily implemented in NGS assays. Another exciting area of cancer research is single-cell analysis. The NGSSR will be heavily involved in strategic planning and design of these experiments. The NGSSR has improved efficiency, created a smooth data pipeline incorporating analyses, and acquired new hardware through multiple funding sources to upgrade its equipment. It continues to provide essential cutting-edge, high-quality, and cost-effective services to MCC users across all 3 scientific programs.

项目摘要-下一代排序共享资源 下一代测序共享资源(NGSSR)的使命是为梅斯癌症中心提供 (MCC)成员拥有最先进的下一代测序技术，用于检测和探索 核苷酸序列改变、DNA拷贝数变异、基因表达差异、染色体 结构变异、染色质修饰和所有与表型复杂性相关的相互作用 出现在癌症中。NGSSR由赵来博士执导，自2012年以来一直是MCC的共享资源。 它位于1,000平方米内。英国《金融时报》格里希儿童癌症研究所(GCCRI)大楼的空间， 毗邻监控中心。赖博士由技术总监Dawn Garcia女士协助。NGSSR进行 为MCC用户在所有3个科学项目中的应用程序排序。服务包括各种类型的图书馆 构建，DNA-seq，RNA-seq，芯片-seq，小RNA-seq和exomeCap-seq，CRISPR筛选，16S 元基因组学和单细胞分析。此外，赖博士和NGSSR团队帮助用户进行实验性 为教职员工和学生设计并提供教育培训。NGSSR与 生物统计与生物信息学共享资源(BBSR)，由生物信息学博士(PSP)陈一东领导 专家。BBSR与NGSSR合作，为MCC成员提供 对NGSSR的产品进行补充。NGSSR由一个科学咨询委员会和MCC监督 共享资源监督委员会(SROC)。2018年，31个同行评审基金使用了NGSSR MCC成员(占总使用量的36%)，并在2018年贡献了12篇与癌症有关的手稿。因为它的 从2014年到2018年年中，NGSSR迅速成为MCC和 改变了MCC成员的基因组研究环境，使 在《新英格兰医学杂志》、《自然》、《癌细胞》、《美国国家癌症研究所》上发表了44篇以上癌症相关出版物的用户数量和投稿情况 以及其他影响较大的同行评议期刊。随着新工具和试剂的开发(例如液体活组织检查) 它们可以很容易地在NGS分析中实施。癌症研究的另一个令人兴奋的领域是单细胞分析。 NGSSR将大力参与这些实验的战略规划和设计。NGSSR已经 提高了效率，创建了包含分析的顺畅数据管道，并通过 多个资金来源以升级其设备。它继续提供必不可少的尖端、高质量、 并在所有3个科学项目中为MCC用户提供经济高效的服务。