New technologies for in situ measurement of exosome release from brain slice cultures

原位测量脑切片培养物中外泌体释放的新技术

• 批准号: 10029256
• 负责人: Christopher A Baker
• 金额: $ 30.23万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-16 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10029256
• 关键词: Biological Assay; Brain; Cells; Circadian Rhythms; Communication; Communities; Complex; Dimensions; Disease; Goals; Human Biology; Immunoassay; In Situ; Lead; Lipids; Measurement; Measures; Mediating; Metabolic Diseases; Microfluidics; Pathology; Perfusion; Research; Role; Sampling; Signal Transduction; Signaling Molecule; Slice; System; Technology; Tissues; Vesicle; base; exosome; extracellular; extracellular vesicles; intercellular communication; nervous system disorder; new technology; new therapeutic target; novel; programs; suprachiasmatic nucleus; tissue culture; tool; vesicular release

Abstract Our research program aims to understand the role of extracellular lipid vesicles in intercellular communication, with the ultimate goal of elucidating new mechanisms of communication involved in disease pathologies. To achieve this, we are developing new bioanalysis tools including tissue culture and perfusion systems for sampling exosomes released from ex vivo tissue slices, and separations-based bioassays for the direct and selective quantitation of exosomes in microfluidic volumes. For the next 5 years, our program goals are: 1) to achieve key system refinements of our tissue culture perfusion system that will enable high sensitivity measurements of secreted factors; 2) to develop a novel mode of separations-based immunoassay tailored specifically to the quantitation of exosome release; and 3) to apply these technologies to investigate a hypothesis of circadian rhythm coordination via extracellular vesicle release in the suprachiasmatic nucleus of the brain. We envision that our research will enable new dimensions of study in the bioanalytical and extracellular vesicle research communities, ultimately leading to new therapies that target dysregulated intercellular communications in neurological and metabolic disorders.

摘要