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Towards noninvasive diagnosis of malaria

迈向疟疾的无创诊断

基本信息

批准号：
10005582
负责人：
Audrey Ragan Odom John
金额：
$ 18.82万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-01-18 至 2021-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005582
关键词：
Africa Aftercare Antimalarials Area Asia Australia Biological Markers Blood Circulation Blood specimen Breath Tests Centers for Disease Control and Prevention (U.S.)Cessation of life Characteristics Child Childhood Clinical Clinical Research Culicidae Data Detection Development Devices Diagnosis Diagnostic Diagnostic tests Enrollment Ensure Exhalation Falciparum Malaria Gene Deletion Global Change Goals HRP-2 protein Health Campaign Infection Interdisciplinary Study Investigation Investments Kenya Laboratory Research Lead Location Machine Learning Malaria Malawi Mass Spectrum Analysis Medical Research Molecular Nose Parasitemia Parasites Parasitology Persons Pilot Projects Plasmodium falciparum Public Health Reporting Reproducibility Research Research Institute Risk Techniques Technology Testing Translating base biomarker discovery breath composition candidate marker clinical Diagnosis cohort cost density diagnostic biomarker experience global health improved innovation malaria infection noninvasive diagnosis pediatric patients portability response sensor specific biomarkers success tool transmission process treatment response volatile organic compound

项目摘要

PROJECT SUMMARY Plasmodium falciparum causes the deadliest form of malaria, which is responsible for nearly one million deaths annually. There is currently no perfect test to detect if a person has malaria. This is because all available tests have challenges and are all based on testing a blood sample. We have identified volatile organic compounds that are produced by the malaria parasite in culture. In a recent pilot study that was conducted in Lilongwe, Ma- lawi, we found that similar compounds are also present in the breath of children who have uncomplicated falci- parum malaria. We also observed a global change in the breath profile of children with malaria. The objectives of this proposal are to identify candidate biomarkers of malaria, with the long-term goal to develop a new non- invasive test to diagnose clinical malaria infection. Supported by our strong preliminary data that indicate that this strategy will be successful, our objectives will be met through two specific aims: 1) determine reproducible changes in the breath composition in response to malaria infection, in an independent location. 2) Relate breath changes with parasite development stages. Our approach is innovative, since we will enroll naturally infected children in the field. Children harbor the highest parasite and gametocyte densities. The proposed re- search is significant, because we will progress in development of new, much-needed malaria diagnostic tool that will not rely on blood samples.

项目摘要恶性疟原虫是最致命的疟疾，造成近100万人死亡。每年。目前还没有完美的测试来检测一个人是否患有疟疾。这是因为所有可用的测试都有挑战性，都是基于对血液样本的测试。我们发现了挥发性有机化合物是由培养的疟原虫产生的。在最近的一项试点研究中，在利隆圭，马- lawi，我们发现类似的化合物也存在于患有单纯性恶性肿瘤的儿童的呼吸中，疟疾。我们还观察到疟疾儿童呼吸特征的全球变化。目标该提案的目的是确定疟疾的候选生物标志物，长期目标是开发一种新的非诊断临床疟疾感染的侵入性测试。我们的初步数据显示，这一战略将是成功的，我们的目标将通过两个具体目标：1）确定可重复的在独立位置，响应疟疾感染的呼吸成分变化。2)有关呼吸随寄生虫发育阶段而变化。我们的方法是创新的，因为我们将自然地注册被感染的孩子在现场。儿童的寄生虫和配子体密度最高。拟议的重新- 研究意义重大，因为我们将在开发新的、急需的疟疾诊断工具方面取得进展不依赖于血液样本

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Volatile profiling distinguishes Streptococcus pyogenes from other respiratory streptococcal species.

挥发性谱分析将化脓性链球菌与其他呼吸道链球菌物种区分开。

DOI：
10.1128/msphere.00194-23
发表时间：
2023-10-24
期刊：
MSPHERE
影响因子：
4.8
作者：
Berna, Amalia Z.;Merriman, Joseph A.;Mellett, Leah;Parchment, Danealle K.;Caparon, Michael G.;Odom John, Audrey R.
通讯作者：
Odom John, Audrey R.

Haloacid Dehalogenase Proteins: Novel Mediators of Metabolic Plasticity in Plasmodium falciparum.

DOI：
10.1177/1178636119848435
发表时间：
2019-01-01
期刊：
Microbiology insights
影响因子：
0
作者：
Frasse, Philip M;Odom John, Audrey R
通讯作者：
Odom John, Audrey R

The Malaria Metabolite HMBPP Does Not Trigger Erythrocyte Terpene Release.

DOI：
10.1021/acsinfecdis.0c00548
发表时间：
2020-10-09
期刊：
ACS infectious diseases
影响因子：
5.3
作者：
Miller JJ;Odom John AR
通讯作者：
Odom John AR

Enzymatic and structural characterization of HAD5, an essential phosphomannomutase of malaria-causing parasites.

DOI：
10.1016/j.jbc.2021.101550
发表时间：
2022-03
期刊：
The Journal of biological chemistry
影响因子：
0
作者：
Frasse PM;Miller JJ;Polino AJ;Soleimani E;Zhu JS;Jakeman DL;Jez JM;Goldberg DE;Odom John AR
通讯作者：
Odom John AR

The Longest Mile: Moving Malaria from Clinical Care to Elimination of Transmission.

最长的一英里：将疟疾从临床护理转向消除传播。