Volatile profiling distinguishes Streptococcus pyogenes from other respiratory streptococcal species.
Volatile profiling distinguishes Streptococcus pyogenes from other respiratory streptococcal species.
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挥发性谱分析将化脓性链球菌与其他呼吸道链球菌物种区分开。
DOI：
10.1128/msphere.00194-23
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发表时间：
2023-10-24
期刊：
影响因子：
4.8
通讯作者：
Odom John, Audrey R.
中科院分区：
文献类型：
--
作者：
Berna, Amalia Z.;Merriman, Joseph A.;Mellett, Leah;Parchment, Danealle K.;Caparon, Michael G.;Odom John, Audrey R.
咽喉痛是门诊最常见的主诉之一。不依赖咽拭子的快速、不依赖培养的诊断技术作为指导门诊抗生素治疗的即时策略将是非常有价值的。尽管这种方法有希望，但在口咽感染期间检测挥发物的努力仍然有限。在我们的研究中，我们试图在体外分离培养物中评估特定细菌挥发性有机化合物（VOC）生物标志物，以便在呼吸生物标志物的初步临床研究之前建立概念验证。化脓性链球菌引起的咽炎诊断的一个特殊挑战是，许多代谢产物可能与链球菌共有。化脓菌和其它相关的口咽定殖细菌种类。因此，我们评估了是否存在足够的代谢差异，以区分A组链球菌的挥发性代谢物组与也定植在呼吸道粘膜的其他链球菌种属，如肺炎链球菌和中间链球菌。在这项工作中，我们确定了27个歧视性的挥发性有机化合物（q值< 0.05），包括醛，醇，含氮化合物，烃，酮，芳香族化合物，酯，醚和羧酸。从这组挥发物中，我们确定了区分S的候选生物标志物。从其他物种的化脓菌，并建立高度生产的VOC，表明存在的S。化脓性链球菌在体外，支持未来的呼吸为基础的诊断测试链球菌性咽炎。在美国，急性咽炎约占1500万次门诊治疗。引起咽炎的最常见和最重要的细菌是化脓性链球菌，占小儿咽炎的15%-30%。在美国，区分细菌性和病毒性咽炎是治疗的关键。通过咽拭子获得的标本培养是咽喉炎微生物学确认的标准实验室程序;然而，这种方法耗时，会延误适当的治疗。如果不治疗，S。化脓性咽炎可导致局部和远处并发症。在这项研究中，我们的特点是挥发性代谢物的S。化脓菌和其它相关的口咽定殖细菌种类。我们确定了区分S的候选生物标志物。其他物种的化脓性链球菌，并提供证据，支持未来的呼吸为基础的诊断测试链球菌性咽炎。
Sore throat is one of the most common complaints encountered in the ambulatory clinical setting. Rapid, culture-independent diagnostic techniques that do not rely on pharyngeal swabs would be highly valuable as a point-of-care strategy to guide outpatient antibiotic treatment. Despite the promise of this approach, efforts to detect volatiles during oropharyngeal infection have yet been limited. In our research study, we sought to evaluate for specific bacterial volatile organic compounds (VOC) biomarkers in isolated cultures in vitro, in order to establish proof-of-concept prior to initial clinical studies of breath biomarkers. A particular challenge for the diagnosis of pharyngitis due to Streptococcus pyogenes is the likelihood that many metabolites may be shared by S. pyogenes and other related oropharyngeal colonizing bacterial species. Therefore, we evaluated whether sufficient metabolic differences are present, which distinguish the volatile metabolome of Group A streptococci from other streptococcal species that also colonize the respiratory mucosa, such as Streptococcus pneumoniae and Streptococcus intermedius. In this work, we identified 27 discriminatory VOCs (q-values < 0.05), composed of aldehydes, alcohols, nitrogen-containing compounds, hydrocarbons, ketones, aromatic compounds, esters, ethers, and carboxylic acid. From this group of volatiles, we identify candidate biomarkers that distinguish S. pyogenes from other species and establish highly produced VOCs that indicate the presence of S. pyogenes in vitro, supporting future breath-based diagnostic testing for streptococcal pharyngitis. Acute pharyngitis accounts for approximately 15 million ambulatory care visits in the United States. The most common and important bacterial cause of pharyngitis is Streptococcus pyogenesis, accounting for 15%–30% of pediatric pharyngitis. Distinguishing between bacterial and viral pharyngitis is key to management in US practice. The culture of a specimen obtained by a throat swab is the standard laboratory procedure for the microbiologic confirmation of pharyngitis; however, this method is time-consuming, which delays appropriate treatment. If left untreated, S. pyogenes pharyngitis may lead to local and distant complications. In this study, we characterized the volatile metabolomes of S. pyogenes and other related oropharyngeal colonizing bacterial species. We identify candidate biomarkers that distinguish S. pyogenes from other species and provide evidence to support future breath-based diagnostic testing for streptococcal pharyngitis.
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