Core B - Biomarkers Core

核心 B - 生物标志物核心

• 批准号: 10005911
• 负责人: Garry R Buettner
• 金额: $ 27.51万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-19 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10005911
• 关键词: Address; Adjuvant; Basic Science; Biochemical; Biochemical Markers; Biological; Biological Assay; Biological Markers; Biological Sciences; Biology; Buffers; Cancer Patient; Cell Culture Techniques; Cell Respiration; Cells; Citric Acid Cycle; Clinical Trials; Communities; Complex; Comprehensive Cancer Center; Core Facility; Couples; Data; Development; Electron Transport; Energy Metabolism; Ensure; Excision; Exposure to; Ferritin; Free Radicals; Genus Hippocampus; Glucose; Goals; Hydrogen Peroxide; Ionizing radiation; Ions; Iowa; Iron; Lead; Liquid substance; Measurement; Medicine; Metabolism; Metals; Methodology; Methods; Mitochondria; Molecular Biology; Monitor; Normal tissue morphology; Oxidation-Reduction; Pathway interactions; Patients; Peroxides; Pharmacologic Ascorbate; Pharmacology Study; Plasma; Pre-Clinical Model; Procedures; Production; Proteins; Reagent; Reproducibility; Research; Research Personnel; Sampling; Scientific Advances and Accomplishments; Services; Source; Standardization; System; Technology; Therapeutic; Time; Tissues; Transferrin; Universities; antioxidant enzyme; ascorbate; cancer therapy; chemoradiation; college; cost effective; data dissemination; dosimetry; experience; experimental study; high standard; improved outcome; innovation; metal transporting protein 1; oxidative damage; pre-clinical; preclinical study; reagent standard; small molecule; synergism; therapy design; tissue injury; tool

Project Summary/Abstract - Core B: The goal of the Biomarkers Core (Core B) is to support Projects 1-3 and Core C in this P01 application by providing bioanalytical support for a broad range of analytical and biochemical assays that address the fundamental redox biology of the production of O2-/H2O2, hydroperoxide metabolism, and processing of redox active metal ions relevant to the study of pharmacological ascorbate (P-AscH-) in preclinical models and clinical trials. Core B will provide validated reagents, e.g. stock solutions of key reagents, standards, Seahorse and EPR analyses, enzymological analyses, ionizing radiation services, and molecular biology reagents, to Projects 1-3. Examples range from our unique, efficient, and cost-effective assays for determining plasma ascorbate concentrations in patient-derived samples (Project 1 and Project 2) as well as assays for ascorbate in the wide variety of samples generated in the proposed preclinical studies (Projects 1-3). Core B has a large repertoire of assays that will examine the basic redox biology of P-AscH-. The leaders of the core have also developed new unique assays and methods that are focused on addressing questions central to the proposed clinical trials in this P01. The leaders have more than 30 years of experience in this field as well as in directing University of Iowa core facilities in the Carver College of Medicine and the Holden Comprehensive Cancer Center. Thus, they are well qualified to lead this core.

项目摘要/摘要 - 核心B： 生物标志物核心（核心B）的目标是通过此P01应用程序支持项目1-3和Core C 为广泛的分析和生化测定法提供生物分析支持，以解决 O2生产的基本氧化还原生物学-/H2O2，氢过氧代谢和氧化还原的加工 与临床前模型中的药物抗坏血酸（P-ASCH-）研究有关的活性金属离子和 临床试验。核心B将提供经过验证的试剂，例如关键试剂，标准的库存解决方案， 海马和EPR分析，酶学分析，电离辐射服务和分子生物学 试剂，1-3项目。示例范围从我们独特，高效且具有成本效益的测定法来确定 患者衍生样品中的血浆抗坏血酸浓度（项目1和项目2）以及测定法 在拟议的临床前研究中产生的各种样品中的抗坏血酸（项目1-3）。核心b 有大量的助手曲目将检查P-Asch-的基本氧化还原生物学。核心领导人 还开发了新的独特测定和方法，这些测定和方法着重于解决核心的问题 在此P01中提出了拟议的临床试验。领导者在这一领域拥有30多年的经验 指导爱荷华大学雕刻师医学院和霍尔顿综合大学的核心设施 癌症中心。那就是他们有资格领导这一核心。