Core B - Biomarkers Core

项目摘要/摘要-核心B： Biomarkers核心(核心B)的目标是通过以下方式支持P01申请中的项目1-3和核心C 为广泛的分析和生化分析提供生物分析支持，以解决 O2、-/H_2O_2产生、氢过氧化氢代谢和氧化还原过程的基本氧化还原生物学 活性金属离子与临床前模型和药物抗坏血酸(P-Asch-)研究有关 临床试验。核心B将提供经过验证的试剂，例如关键试剂的库存解决方案、标准、 海马和EPR分析、酶分析、电离辐射服务和分子生物学 试剂，到项目1-3。例如，我们独特、高效且经济实惠的分析方法可用于确定 患者来源样本中的血浆抗坏血酸浓度(项目1和项目2)以及 在拟议的临床前研究(项目1-3)中产生的各种样本中的抗坏血酸。核心B 有大量的分析方法，将检查P-Asch-的基本氧化还原生物学。核心国家的领导人 还开发了新的独特的分析和方法，专注于解决 本P01中建议的临床试验。领导者在这一领域拥有30多年的经验，以及 在指导爱荷华州大学卡弗医学院和霍尔顿综合医院的核心设施方面 癌症中心。因此，他们完全有资格领导这个核心。

Project Summary/Abstract - Core B: The goal of the Biomarkers Core (Core B) is to support Projects 1-3 and Core C in this P01 application by providing bioanalytical support for a broad range of analytical and biochemical assays that address the fundamental redox biology of the production of O2-/H2O2, hydroperoxide metabolism, and processing of redox active metal ions relevant to the study of pharmacological ascorbate (P-AscH-) in preclinical models and clinical trials. Core B will provide validated reagents, e.g. stock solutions of key reagents, standards, Seahorse and EPR analyses, enzymological analyses, ionizing radiation services, and molecular biology reagents, to Projects 1-3. Examples range from our unique, efficient, and cost-effective assays for determining plasma ascorbate concentrations in patient-derived samples (Project 1 and Project 2) as well as assays for ascorbate in the wide variety of samples generated in the proposed preclinical studies (Projects 1-3). Core B has a large repertoire of assays that will examine the basic redox biology of P-AscH-. The leaders of the core have also developed new unique assays and methods that are focused on addressing questions central to the proposed clinical trials in this P01. The leaders have more than 30 years of experience in this field as well as in directing University of Iowa core facilities in the Carver College of Medicine and the Holden Comprehensive Cancer Center. Thus, they are well qualified to lead this core.