权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Bioenergetics Core

生物能核心

基本信息

批准号：
10005395
负责人：
Craig Cano Beeson
金额：
$ 15.32万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-01 至 2022-01-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005395
关键词：
Amino Acids Atmosphere Bicarbonates Bioenergetics Biological Assay Biological Models Biological Sciences Biology Carbon Dioxide Cardiac Cell Line Cell physiology Cells Cellular biology Centers of Research Excellence Complex Core Facility Coupling DNA Data Development Disease Disease model Doctor of Philosophy Electrons Embryo Energy Metabolism Engineering Equilibrium Equus caballus Failure Fatty Acids Fluorescent Probes Gases Genetic Genus Hippocampus Glycolysis Goals Gold Head Heart Heart Valves Heterogeneity Hydration status Image Image Analysis Impairment Intervention Kidney Lactic acid Lead Liver Measurement Measures Membrane Potentials Metabolic Methods Mitochondria Mus Neurons Nutrient Organelles Oxidants Oxidation-Reduction Oxidative Stress Oxygen Consumption Pathologic Pathway interactions Peptides Pharmacology Phase Phosphorylation Physiology Play Production Protons Reaction Reactive Oxygen Species Regulation Renal tubule structure Research Personnel Research Support Resources Respiration Role Running Sample Size Sampling Science Services Signal Transduction Site Slice Sodium Chloride Source South Carolina Stream Stress Structure System Techniques Technology Time Tissue Sample Tissues Translations Validation Work Zebrafish anaerobic glycolysis base cell immortalization cost design drug discovery established cell line experimental study extracellular gene therapy improved in vivo innovation instrument metabolomics mitochondrial membrane monolayer oxidation professor programs protein function sensor success sugar synergism technology validation therapeutic target three dimensional cell culture time use tool tumor

项目摘要

CORE C: Bioenergetics Profiling Core Director: Craig Cano Beeson, Ph.D. Professor, Department of Drug Discovery and Biomedical Sciences Project Summary The primary goal of the Bioenergetics Profiling core facility is to support COBRE investigators in the characterization of metabolite fluxes related to cellular redox and primary energy metabolism – the prevalent sources of both the primary and secondary cellular redox species. The facility provides access to traditional, `gold standard' techniques such as isotopomer, radiometric, and spectroscopic analyses. The core is also a development site for the Seahorse Biosciences extracellular flux (XF) fluorometric technology used to measure metabolic fluxes (i.e., oxygen consumption, CO2 and lactate extrusion) in real time using multiwell plates. The basic Seahorse XF applications enable high throughput metabolic measurements with small sample sizes that have transformed the utility of quantitative analyses of metabolic fluxes. Innovative adaptations of the XF technologies developed in the core facility are providing access to real time flux measurements of redox species in cells and tissues and, more importantly, the interrogation of bioenergetics pathways via use of pharmacological or genetic interventions. We have coordinated these recent strategic technological acquisitions into a core that provides analytical support for the efforts of the COBRE investigators and their collaborators while also extending the technology to suit new efforts and further enable measurements with improved translational potential. In the COBRE Phase I stage the core was known as the “Metabolomics Core” that offered nascent Seahorse XF technology in addition to traditional single time-point quantification of metabolite concentrations via LC-MS or NMR techniques. As described below, these traditional metabolomics techniques were not as useful to the lead COBRE investigators as were the XF technologies, particularly as the latter technology advanced and traditional metabolomics has matured. Because traditional metabolomics analyses are now commercially available leading to cost reductions (much as academic DNA oligomer or peptide cores became obsolete), the metabolomics core has been renamed as the “Bioenergetics Profiling Core” to reflect the XF services we have developed that best support our investigators, and for which there are no alternative external resources. Indeed, because XF technologies utilize living samples, it is unlikely they will be easily `out-sourced'.

核心C：生物能量分析核心